ANTI-HUMAN CD38 (HIT2) PE-Cyanine5
货号：15-0389-42
规格：100TESTS
价格：2629
产品类型：流式抗体
品牌：eBioscience
物种：人
宿主：小鼠
抗体亚型：IgG
荧光染料：PE-Cy5
| 抗体类型: | 单抗 | 同型对照： | IgG1, kappa |
| 免疫原性： | | 用法： | 5uL（0.125µg）/test(Flow） |
| Description: The HIT2 monoclonal antibody reacts with the human CD38 molecule, an approximately 45 kDa type II transmembrane protein. CD38 is expressed by thymocytes, peripheral B cells including plasma cells, activated T cells, and monocytes. CD38 is a counter-receptor for CD31 and has both cyclase and hydrolase enzymatic activity.Applications Reported: The HIT2 antibody has been reported for use in flow cytometric analysis.Applications Tested: This HIT2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered. |
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| 参考文献： |
| 1.The Journal of biological chemistrySuppression of homeobox transcription factor VentX promotes expansion of human hematopoietic stem/multipotent progenitor cells."15-0389 was used in Flow cytometry/Cell sorting to study the mechanisms that regulate the expansion of human hematopoietic stem/multipotent progenitor cells, showing that VentX regulates critical cell cycle regulators and Wnt downstream genes previously implicated in HSC/MPP proliferation and expansion."Authors Gao H,Wu X,Sun Y,Zhou S,Silberstein LE,Zhu Z2.The Biochemical journalCD38 binding to human myeloid cells is mediated by mouse and human CD31.Authors Horenstein AL,Stockinger H,Imhof BA,Malavasi F |
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