ANTI-HUMAN CD38 (HIT2)Alexa Fluor 700

2024-10-17

ANTI-HUMAN CD38 (HIT2)Alexa Fluor 700

货号:56-0389-41,56-0389-42

规格:25TESTS,100TESTS

价格:2447,3107

产品类型:流式抗体

品牌:eBioscience

抗原:CD38

物种:人

宿主:小鼠

抗体亚型:IgG

克隆号:HIT2

荧光染料:Alexa Fluor 700

抗体类型:单抗同型对照:IgG1, kappa
免疫原性:用法:5uL(0.5µg)/test(Flow)
Description: The HIT2 monoclonal antibody reacts with the human CD38 molecule, an approximately 45 kDa type II transmembrane protein. CD38 is expressed by thymocytes, peripheral B cells including plasma cells, activated T cells, and monocytes. CD38 is a counter-receptor for CD31 and has both cyclase and hydrolase enzymatic activity.Applications Reported: This HIT2 antibody has been reported for use in flow cytometric analysis.Applications Tested: This HIT2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Alexa Fluor® 700 emits at 723 nm and can be excited with the red laser (633 nm). Most instruments will require a 685 LP mirror and 710/20 filter. Please make sure that your instrument is capable of detecting this fluorochrome.Excitation: 633-647 nm; Emission: 723 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.
数据

CD38 Antibody (56-0389-41) in FlowStaining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control Alexa Fluor® 700 (Product # 56-4714-80) (open histogram) or Anti-Human CD38 Alexa Fluor® 700 (filled histogram). Cells in the lymphocyte (left) and monocyte (right) gates were used for analysis.
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参考文献:
1.Genome biologySingle-cell profiling of human megakaryocyte-erythroid progenitors identifies distinct megakaryocyte and erythroid differentiation pathways."56-0389 was used in Flow cytometry/Cell sorting to clarify the cellular hierarchy in human megakaryocyte/erythroid lineage commitment and highlight the importance of using a combination of single-cell approaches to dissect cellular heterogeneity."Authors Psaila B,Barkas N,Iskander D,Roy A,Anderson S,Ashley N,Caputo VS,Lichtenberg J,Loaiza S,Bodine DM,Karadimitris A,Mead AJ,Roberts I2.AgingA novel autosomal recessive TERT T1129P mutation in a dyskeratosis congenita family leads to cellular senescence and loss of CD34+ hematopoietic stem cells not reversible by mTOR-inhibition."56-0389 was used in Flow cytometry/Cell sorting to outline limitations of a potential dyskeratosis congenita therapy based on rapamycin."Authors Stockklausner C,Raffel S,Klermund J,Bandapalli OR,Beier F,Brümmendorf TH,Bürger F,Sauer SW,Hoffmann GF,Lorenz H,Tagliaferri L,Nowak D,Hofmann WK,Buergermeister R,Kerber C,Rausch T,Korbel JO,Luke B,Trumpp A,Kulozik AE3.Frontiers in immunologyInhibitory Receptor Expression Depends More Dominantly on Differentiation and Activation than "Exhaustion" of Human CD8 T Cells."56-0389 was used in Flow cytometry/Cell sorting to evaluate the direct relationship between inhibitory receptor expression and functionality in CD8 T cells."Authors Legat A,Speiser DE,Pircher H,Zehn D,Fuertes Marraco SA

技术参数

产品应用 Flow

细胞类型 HSC

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