ANTI-HUMAN CD45RA (JS-83) FITC

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ANTI-HUMAN CD45RA (JS-83) FITC

货号:11-9979-41,11-9979-42

规格:25TESTS,100TESTS

价格:807,1924

产品类型:流式抗体

品牌:eBioscience

抗原:CD45RA

物种:人

宿主:小鼠

抗体亚型:IgG

克隆号:JS-83

荧光染料:FITC

抗体类型:单抗同型对照:IgG1, kappa
免疫原性:用法:5uL(0.125 µg)/test(Flow)
Description: The JS-83 monoclonal antibody reacts with human CD45RA, a 220 kDa molecule expressed by subpopulations of CD4^+ peripheral T lymphocytes, CD8^+ peripheral T lymphocytes, and B cells. The CD45RA^+ T cell populations are mainly naive/virgin allowing the use of JS-83 mAb as a phenotypic marker to discriminate T cell subsets.Applications Reported: JS-83 has been reported for use in flow cytometric analysis.Applications Tested: This JS-83 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.
数据

CD45RA Antibody (11-9979-41) in FlowNormal human peripheral blood cells were stained with Mouse IgG1 K Isotype Control FITC (Product # 11-4714-42) (open histogram) or Anti-Human CD45RA FITC (filled histogram). Cells in the lymphocyte population were used for analysis.

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参考文献:
1.JCI insightB cells from African American lupus patients exhibit an activated phenotype."11-9979 was used in Flow cytometry/Cell sorting to investigate immunological differences between African American and European American patients with systemic lupus erythematosus, showing that B cells from African American patients exhibit an activated phenotype."Authors Menard LC,Habte S,Gonsiorek W,Lee D,Banas D,Holloway DA,Manjarrez-Orduno N,Cunningham M,Stetsko D,Casano F,Kansal S,Davis PM,Carman J,Zhang CK,Abidi F,Furie R,Nadler SG,Suchard SJ2.Immunology and cell biologyProtective genotypes in HIV infection reflect superior function of KIR3DS1+ over KIR3DL1+ CD8+ T cells."11-9979 was used in Flow cytometry/Cell sorting to investigate how HLA/KIR genotypic combinations relate to the phenotype and function of CD8(+) T cells in individuals with HIV infection."Authors Zipperlen K,Gallant M,Stapleton S,Heath J,Barrett L,Grant M3.BMC immunologyDivergent adaptive and innate immunological responses are observed in humans following blunt trauma."11-9979 was used in Flow cytometry/Cell sorting to suggest that trauma is followed by hyper- and hypo-inflammatory responses from the innate and adaptive immune system respectively."Authors Kasten KR,Goetzman HS,Reid MR,Rasper AM,Adediran SG,Robinson CT,Cave CM,Solomkin JS,Lentsch AB,Johannigman JA,Caldwell CC

技术参数

产品应用 Flow

细胞类型 HSC

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