| Description: The HI100 monoclonal antibody reacts with human CD45RA, a 220 kDa molecule expressed by subpopulations of CD4+ peripheral T lymphocytes, CD8+ peripheral T lymphocytes, and B cells. The CD45RA+ T cell populations are mainly naive/virgin allowing the use of HI100 mAb as a phenotypic marker to discriminate T cell subsets.Applications Reported: The HI100 antibody has been reported for use in flow cytometric analysis.Applications Tested: This HI100 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered. |
| 1.The Journal of experimental medicineCirculating precursors of human CD1c+ and CD141+ dendritic cells."12-0458 was used in Flow cytometry/Cell sorting to identify a migratory precursor of dendritic cells using a novel tissue culture system."Authors Breton G,Lee J,Zhou YJ,Schreiber JJ,Keler T,Puhr S,Anandasabapathy N,Schlesinger S,Caskey M,Liu K,Nussenzweig MC2.The Journal of experimental medicineRestricted dendritic cell and monocyte progenitors in human cord blood and bone marrow."12-0458 was used in Flow cytometry/Cell sorting to study the development of dendritic cells from hematopoietic stem cell progenitors using a novel culture system."Authors Lee J,Breton G,Oliveira TY,Zhou YJ,Aljoufi A,Puhr S,Cameron MJ,Sékaly RP,Nussenzweig MC,Liu K3.Molecular medicine reportsSkewed T-helper (Th)1/2- and Th17/T regulatory‑cell balances in patients with renal cell carcinoma."12-0458 was used in Flow cytometry/Cell sorting to demonstrate that the balance of Th1 and Th2 cells is skewed towards the Th2 profile in the peripheral blood of patients with RCC."Authors Li L,Yang C,Zhao Z,Xu B,Zheng M,Zhang C,Min Z,Guo J,Rong R4.Scientific reportsProportions of CD4+ memory T cells are altered in individuals chronically infected with Schistosoma haematobium."12-0458 was used in Flow cytometry/Cell sorting to investigate the association between human helminth infection and CD4+ memory T cell proportion in individuals chronically infected with Schistosoma haematobium."Authors Nausch N,Bourke CD,Appleby LJ,Rujeni N,Lantz O,Trottein F,Midzi N,Mduluza T,Mutapi F5.PloS oneThe acute environment, rather than T cell subset pre-commitment, regulates expression of the human T cell cytokine amphiregulin."12-0458 was used in Flow cytometry/Cell sorting to collate a detailed analysis of the regulation of Amphiregulin expression by human T cell subsets."Authors Qi Y,Operario DJ,Georas SN,Mosmann TR |
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