ANTI-HUMAN CD45RA (HI100) PE-Cyanine5
货号：15-0458-41,15-0458-42
规格：25TESTS,100TESTS
价格：0,2629
产品类型：流式抗体
品牌：eBioscience
抗原：CD45RA
物种：人
宿主：小鼠
抗体亚型：IgG
克隆号：HI100
荧光染料：PE-Cy5
| 抗体类型: | 单抗 | 同型对照： | IgG2b, kappa |
| 免疫原性： | | 用法： | 5uL（0.125 µg）/test(Flow） |
| Description: The HI100 monoclonal antibody reacts with human CD45RA, a 220 kDa molecule expressed by subpopulations of CD4+ peripheral T lymphocytes, CD8+ peripheral T lymphocytes, and B cells. The CD45RA+ T cell populations are mainly naive/virgin allowing the use of HI100 mAb as a phenotypic marker to discriminate T cell subsets.Applications Reported: The HI100 antibody has been reported for use in flow cytometric analysis.Applications Tested: This HI100 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered. |
| 数据 |
| CD45RA Antibody (15-0458-41) in FlowStaining of normal human peripheral blood cells with Mouse IgG2b K Isotype Control PE-Cy5 (Product # 15-4732-42) (blue histogram) or Anti-Human CD45RA PE-Cyanine5 (purple histogram).Cells in the lymphocyte gate were used for analysis. |
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