6x-His Tag Monoclonal Antibody (HIS.H8)-Bio-Rad-Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱

6x-His Tag Monoclonal Antibody (HIS.H8)

2024-10-17

6x-His Tag Monoclonal Antibody (HIS.H8)

货号：MA121315

规格：100 µg

价格：2547

产品类型：标签抗体

品牌：Thermo Fisher

物种：其它

宿主：小鼠

抗体亚型：IgG2b, kappa

克隆号：HIS.H8

荧光染料：其它

抗体类型:	单抗	同型对照：	lgG2b
免疫原性：	6x His synthetic peptide	用法：	Assay dependent(ELISA)； 1:500-1:2000(ICC)； 1:500 - 1:2000(IF) ； Assay dependent(IP)； 1:1000 - 1:3000(WB)
Product Specific Information MA1-21315 detects 6X-His Epitope Tag in samples.MA1-21315 has been successfully used in ELISA, immunofluorescence, immunoprecipitation and Western Blot procedures.MA1-21315 immunogen corresponds to 6x His synthetic peptide.MA1-21315 may be stored at 4°C short term. Add 0.05% sodium azide if desired. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Background/Target InformationThe 6x His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH). The His tag is commonly expressed as a tag at either N-or C-terminal regions of recombinant proteins to allow isolation or purification by immobilized metal affinity chromatography. Epitope tagging is a technique in which a known epitope is fused to a recombinant protein using genetic engineering. By choosing a particular epitope and recombinant protein combination, epitope tagging makes it possible to detect proteins for which no antibody is available. His epitope tagged proteins that contain a stretch of histidine residues at the carboxyl or amino terminus are used for purification of recombinant proteins by means of metal chelate chromatography due to metal binding properties of histidine clusters on His tagged proteins. For example, the affinity of the histidine-tag motif to Ni2+ by chelation is strong and selective enough to enable purification of the protein to homogeneity by affinity chromatography on a Ni2+-NTA adsorbent. His tag-specific antibodies are used to facilitate detection or coimmunopreciptation of tagged proteins.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
数据
6x-His Tag Antibody (MA1-21315) in IFLEFT: untransfected control; RIGHT: anti-His (in red) on His-tagged fusion proteins in HEK293 cells. Both counterstained with DAPI (in blue)
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参考文献：
1.mBio Translation Stress Positively Regulates MscL-Dependent Excretion of Cytoplasmic Proteins.2018.Authors Morra R,Del Carratore F,Muhamadali H,Horga LG,Halliwell S,Goodacre R,Breitling R,Dixon N 2.PLoS genetics An inhibitory mono-ubiquitylation of the Drosophila initiator caspase Dronc functions in both apoptotic and non-apoptotic pathways.2017.View published figure on By clicking the link above, you will be redirected to the Benchsci website. Disclaimer 3.Frontiers in microbiology High Protective Efficacy of Probiotics and Rice Bran against Human Norovirus Infection and Diarrhea in Gnotobiotic Pigs.2017.View published figure on By clicking the link above, you will be redirected to the Benchsci website. Disclaimer 4.Protein expression and purification Convenient expression, purification and quantitative liquid chromatography-tandem mass spectrometry-based analysis of TET2 5-methylcytosine demethylase.2017.Authors Jaiswal M,Bhar S,Vemula H,Prakash S,Ponnaluri VKC,Gutheil WG,Mukherji M 5.Journal of virology The Human Cytomegalovirus IE1 Protein Antagonizes PML Nuclear Body-Mediated Intrinsic Immunity via the Inhibition of PML De Novo SUMOylation.2017. Authors Schilling EM,Scherer M,Reuter N,Schweininger J,Muller YA,Stamminger T

技术参数

产品应用 WB；IF；Flow；IP；ELSA；ICC

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6x-His Tag Monoclonal Antibody (HIS.H8)

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