CD44 Monoclonal Antibody (IM7), FITC

货号：11-0441-81,11-0441-82,11-0441-85,11-0441-86

规格：50 µg,100 µg,500 µg,1 mg

价格：750,1358,1255,4824

产品类型：流式抗体

品牌：eBioscience

抗原：CD44

物种：人/小鼠

宿主：大鼠

抗体亚型：IgG2b, kappa

克隆号：IM7

荧光染料：FITC

类型:	单抗	同型对照：	Rat IgG2b kappa Isotype Control, FITC, eBioscience™
浓度：	0.5 mg/mL	用法：	0.5 µg/tests(Flow)

产品详细信息Description: The IM7 monoclonal antibody reacts with all isoforms of mouse CD44 (Pgp-1). CD44 is expressed by hematopoietic and non-hematopoietic cells. Bone marrow myeloid cells and memory T cells highly express this antigen and peripheral B and T cells can upregulate the expression of CD44. CD44 functions as an adhesion molecule through its binding to hyaluronate, an extracellular matrix component.Applications Reported: The IM7 antibody has been reported for use in flow cytometric analysis.Applications Tested: The IM7 antibody has been tested by flow cytometric analysis of mouse bone marrow cells and splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD44 cell surface antigen is a 100 kDa type 1 transmembrane glycoprotein widely expressed on human leucocytes, white matter of the brain and by some epithelial cells of the intestine and breast. Several isoforms of CD44 exist, including the predominant CD44H isoform detected in many normal tissues. CD44 is a receptor for hyaluronic acid (HA) and is involved in cell-cell interactions, cell adhesion and migration. CD44 also participates in a wide variety of cellular functions including lymphocyteactivation, recirculation and homing. CD44 expression may be up-regulated upon some carcinomas, and it has been speculated that this may be related to metastatic potential. CD44 is expressed by hematopoietic, non-hematopoietic cells, epithelial tissues, and to filopodia in cultured keratinocytes. Further, bone marrow myeloid cells and memory T cells express CD44 at high levels, and peripheral B and T cells can upregulate the expression of CD44 in response to certain stimulatory events. Transcripts for the CD44 gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full-length nature of some of these variants have not been determined. Alternative splicing is the basis for the structural and functional diversity of the CD44 protein. Diseases associated with CD44 dysfunction include superficial keratitis and lichen sclerosus. CD44 also may be related to tumor metastasis formation.

数据

CD44 Antibody (11-0441-81) in FlowStaining of C57BL/6 splenocytes with 0.25 µg of Rat IgG2b kappa Isotype Control FITC(Product # 11-4031-82) (open histogram) or 0.25 µg of Anti-Human/Mouse CD44 FITC(filled histogram). Total viable cells were used for analysis.

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参考文献：

1.OncoimmunologyT cells."11-0441 was used in Flow cytometry/Cell sorting to reveal a tumor-inhibiting role for LYG1 through promoting the activation, proliferation, and function of CD4+T cells in antitumor immune responses, offering implications for novel tumor immunotherapy."Authors Liu H,Zhang Y,Liu Z,Wang P,Mo X,Fu W,Liu W,Cheng Y,Han W2.The Journal of clinical investigationExcessive expression of miR-27 impairs Treg-mediated immunological tolerance.View published figure on By clicking the link above, you will be redirected to the Benchsci website. Disclaimer3.EBioMedicineStructure-guided development of a high-affinity human Programmed Cell Death-1: Implications for tumor immunotherapy."11-0441 was used in Flow cytometry/Cell sorting to highlight the value of structural considerations in the design of a PD-1 Ig fusion protein with immune modulatory properties."Authors Lázár-Molnár E,Scandiuzzi L,Basu I,Quinn T,Sylvestre E,Palmieri E,Ramagopal UA,Nathenson SG,Guha C,Almo SC4.Scientific reportsAn attenuated Mycobacterium tuberculosis clinical strain with a defect in ESX-1 secretion induces minimal host immune responses and pathology."11-0441 was used in Flow cytometry/Cell sorting to investigate the Mycobacterium tuberculosis (M.tb) DK9897 strain, showing that it is attenuated and has a defect in ESX-1 secretion, leading to minimal host immune response and pathology."Authors Clemmensen HS,Knudsen NPH,Rasmussen EM,Winkler J,Rosenkrands I,Ahmad A,Lillebaek T,Sherman DR,Andersen PL,Aagaard C5.The Journal of experimental medicineDC subset-specific induction of T cell responses upon antigen uptake via Fcγ receptors in vivo."11-0441 was used in Flow cytometry/Cell sorting to suggest that targeting antigens to FcγRs on DCs may be a promising approach to raise immunogenic or tolerogenic T cell responses to antigens of choice."Authors Lehmann CHK,Baranska A,Heidkamp GF,Heger L,Neubert K,Lühr JJ,Hoffmann A,Reimer KC,Brückner C,Beck S,Seeling M,Kießling M,Soulat D,Krug AB,Ravetch JV,Leusen JHW,Nimmerjahn F,Dudziak D

技术参数

产品应用 Flow；ICC；IF；WB

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