CD137 Ligand (4-1BB Ligand) Monoclonal Antibody (4H3), PerCP-eFluor 710
货号：46-5906-42
规格：100 Tests
价格：3416
产品类型：流式抗体
品牌：Thermo Fisher
物种：人
宿主：小鼠
抗体亚型：IgG1, kappa
荧光染料：PerCP-eFluor™ 710
| 抗体类型 | 单抗 | 同型对照： | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PerCP-eFluor 710 |
| 浓度： | 5 µL/Test | 用法： | 5 μL (0.06 μg)/test (Flow) |
| 产品详细信息This 4H3 monoclonal antibody recognizes human CD137 ligand, also known as 4-1BB ligand, TNFSF9, or CDw137L.Applications Reported: This 4H3 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 4H3 antibody has been pre-diluted and tested by flow cytometric analysis of stimulated normal human peripheral blood cells. This may be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.PerCP-eFluor™ 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD137 ligand (CD137L) is a type II membrane protein and part of the TNF superfamily. CD137L is a co-stimulatory molecule that is expressed on antigen presenting cells (DCs, monocytes/macrophages, B cells) and is upregulated upon activation. Its receptor, CD137/4-1BB, is found on a variety of cells, including inflamed endothelial cells, where its expression enhances extravasation of CD137L expressing monocytes. Activated T cells also express CD137, and engagement with CD137L enhances T cell proliferation, IL-2 secretion, survival, and cytotoxic activity. CD137L is also expressed on a majority of B-cell lymphomas, including mantle cell lymphoma, follicular lymphoma, and diffuse large B-cell lymphoma (but it is lacking on Hodgkin lymphoma and T-cell lymphoma). CD137L is also expressed in carcinoma cell lines, and is thought to be involved in T cell-tumor cell interactions. |
| 数据 |
| CD137 Ligand (4-1BB Ligand) Antibody (46-5906-42) in FlowNormal human peripheral blood cells were unstimulated (left) or stimulated with 10 µg/mL of F (ab’)2 Anti-Human IgM+IgG Functional Grade Purified (Product # 16-5099-85) and 3 µg/mL CD40 Functional Grade Monoclonal Antibody (Product # 16-0409-81) (right) for 4 hours. Cells were surface stained with Anti-Human CD19 APC (Product # 17-0191-82), Anti-Human CD69 eFluor® 450 (Product # 48-0699-42), and Anti-Human CD137 Ligand (4-1BB Ligand) PerCP-eFluor 710. CD19 positive cells in the lymphocyte gate were used for analysis. |
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