SSEA1 Monoclonal Antibody (eBioMC-480 (MC-480)), Biotin, eBioscience™

货号：13-8813-82

规格：100 µg

价格：2969

产品类型：流式抗体

品牌：eBioscience

抗原：SSEA1

物种：人/小鼠

宿主：小鼠

抗体亚型：IgM

克隆号：eBioMC-480 (MC-480)

荧光染料：Biotin

抗体类型:	单抗	同型对照：	IgM
免疫原性：		用法：	05 µg/test(Flow）

Description: The eBioMC-480 (MC-480) antibody reacts with the stage-specific embryonic antigen-1 (SSEA-1), a carbohydrate epitope expressed upon the surface of early mouse embryos, murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES) and murine and human germ cells (EG). No immunoreactivity is evident with undifferentiated human EC and ES cells. Differentiation of human EC results in an increase in SSEA-1 expression, while in the mouse expression is diminished. Expression of the carbohydrate moiety is also found on mature human granulocytes (on CD15) and some monocytes. SSEA-1 is associated with cell adhesion, migration and differentiation.Applications Reported: This eBioMC-480 (MC-480) antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBioMC-480 (MC-480) antibody has been tested by flow cytometric analysis of the F9 cell line. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationCD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells. There are several variants of Lewis x, such as sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkin and acutes disease.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

SSEA1 Antibody (13-8813-82) in FlowStaining of the F9 cell line with staining buffer (autofluorescence) (open histogram) or 0.25 µg of Anti-Human/Mouse SSEA-1 Biotin (filled histogram)followed by Streptavidin PE (Product # 12-4317-87). Total viable cells were used for analysis.

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参考文献：

1.Stem cells (Dayton, Ohio)Plant Homeo Domain Finger Protein 8 Regulates Mesodermal and Cardiac Differentiation of Embryonic Stem Cells Through Mediating the Histone Demethylation of pmaip1."13881382 was used in immunocytochemistry to study the role of PHF8 during mesodermal and cardiac lineage commitment using mouse embryonic stem cells"Authors Tang Y,Hong YZ,Bai HJ,Wu Q,Chen CD,Lang JY,Boheler KR,Yang HT2.Stem cell reportsAlternative Routes to Induced Pluripotent Stem Cells Revealed by Reprogramming of the Neural Lineage.View published figure on By clicking the link above, you will be redirected to the Benchsci website. Disclaimer3.OncotargetCoexpression analysis of CD133 and CD44 identifies proneural and mesenchymal subtypes of glioblastoma multiforme."13-8813 was used in Flow cytometry/Cell sorting to identify two new potential protein markers for molecular glioma subtype."Authors Brown DV,Daniel PM,D'Abaco GM,Gogos A,Ng W,Morokoff AP,Mantamadiotis T4.NatureParent stem cells can serve as niches for their daughter cells.View published figure on By clicking the link above, you will be redirected to the Benchsci website. Disclaimer5.Nature cell biologyEmbryonic stem cells require Wnt proteins to prevent differentiation to epiblast stem cells.View published figure on By clicking the link above, you will be redirected to the Benchsci website. Disclaimer

技术参数

产品应用 Flow；ICC；IHC；IF

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