CD140a (PDGFRA) Monoclonal Antibody (APA5), PE, eBioscience™

货号：12-1401-81

规格：50 µg

价格：1728

产品类型：流式抗体

品牌：eBioscience

抗原：CD140a（PDGFRA）

物种：人/小鼠

宿主：大鼠

抗体亚型：IgG

克隆号：APA5

荧光染料：PE

抗体类型:	单抗	同型对照：	IgG2a, kappa
免疫原性：		用法：	0.5 µg/test(Flow）

Description: The APA5 monoclonal antibody reacts with the mouse CD140a molecule, the alpha chain of the platelet derived growth factor receptor (PDGF receptor). PDGFRa is a receptor tyrosine kinase that forms dimers on the surface upon ligand binding and phosphorylates substrates. Dimers of PDGFR consist of either homodimers of alpha/alpha, beta/beta, or heterodimers of alpha/beta and serve as a substrate for its kinase activity. CD140a is expressed by embryonic tissues and mesenchymal-derived cells of the adult mouse tissues.Applications Reported: This APA5 antibody has been reported for use in flow cytometric analysis.Applications Tested: This APA5 antibody has been tested by flow cytometric analysis of NIH/3T3 cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationPDGFRA is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. Studies in knockout mice, where homozygosity is lethal, indicate that the alpha form of the platelet-derived growth factor receptor is particularly important for kidney development since mice heterozygous for the receptor exhibit defective kidney phenotypes.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

CD140a (PDGFRA) Antibody (12-1401-81) in FlowStaining of NIH/3T3 cells with 0.5 µg of Rat IgG2a K Isotype Control PE(Product # 12-4321-80) (blue histogram) or 0.5 µg of Anti-Mouse CD140a (PDGF Receptor a)PE (purple histogram). Total viable cells were used for analysis.

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参考文献：

1.Cell stem cellAdipocyte Accumulation in the Bone Marrow during Obesity and Aging Impairs Stem Cell-Based Hematopoietic and Bone Regeneration."12-1401 was used in Flow cytometry/Cell sorting to specify the cellular identities of the adipogenic and osteogenic lineages of the bone."Authors Ambrosi TH,Scialdone A,Graja A,Gohlke S,Jank AM,Bocian C,Woelk L,Fan H,Logan DW,Schürmann A,Saraiva LR,Schulz TJ2.Cell cycle (Georgetown, Tex.)Generation of embryonic stem cells from mouse adipose-tissue derived cells via somatic cell nuclear transfer."12-1401 was used in Flow cytometry/Cell sorting to demonstrates that ESCs can be generated from the adipose tissue by somatic cell nuclear transfer (SCNT) and suggests that ADCs can be a new donor-cell type for potential therapeutic cloning."Authors Qin Y,Qin J,Zhou C,Li J,Gao WQ3.Nature medicineLoss of fibronectin from the aged stem cell niche affects the regenerative capacity of skeletal muscle in mice."12140181 was used in flow cytometry to show that the loss of stem cell adhesion to fibronectin is a mechanism of aging"Authors Lukjanenko L,Jung MJ,Hegde N,Perruisseau-Carrier C,Migliavacca E,Rozo M,Karaz S,Jacot G,Schmidt M,Li L,Metairon S,Raymond F,Lee U,Sizzano F,Wilson DH,Dumont NA,Palini A,Fässler R,Steiner P,Descombes P,Rudnicki MA,Fan CM,von Maltzahn J,Feige JN,Bentzinger CF4.Development (Cambridge, England)ER71 directs mesodermal fate decisions during embryogenesis.View published figure on By clicking the link above, you will be redirected to the Benchsci website. Disclaimer5.Proceedings of the National Academy of Sciences of the United States of AmericaOlig2+ neuroepithelial motoneuron progenitors are not multipotent stem cells in vivo.Authors Mukouyama YS,Deneen B,Lukaszewicz A,Novitch BG,Wichterle H,Jessell TM,Anderson DJ

技术参数

产品应用 Flow；HC (P)；IHC (F)

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