CD140a (PDGFRA) Monoclonal Antibody (APA5), Super Bright 702, eBioscience™
货号：67-1401-80,67-1401-82
规格：25 µg ,100 µg
价格：2371,3345
产品类型：流式抗体
品牌：eBioscience
抗原：CD140a (PDGFRA)
物种：小鼠
宿主：大鼠
抗体亚型：IgG
克隆号：APA5
荧光染料：Super Bright 702
| 抗体类型: | 单抗 | 同型对照： | IgG2a, kappa |
| 免疫原性： | | 用法： | 1.0 µg/test(Flow） |
Description: The APA5 monoclonal antibody reacts with the mouse CD140a molecule, the alpha chain of the platelet derived growth factor receptor (PDGF receptor). PDGFRa is a receptor tyrosine kinase that forms dimers on the surface upon ligand binding and phosphorylates substrates. Dimers of PDGFR consist of either homodimers of alpha/alpha, beta/beta, or heterodimers of alpha/beta and serve as a substrate for its kinase activity. CD140a is expressed by embryonic tissues and mesenchymal-derived cells of the adult mouse tissues.Applications Reported: This APA5 antibody has been reported for use in flow cytometric analysis.Applications Tested: This APA5 antibody has been tested by flow cytometric analysis of NIH/3T3 cells. This may be used at less than or equal to 1.0 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Super Bright 702 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 702 nm. We recommend using a 710/50 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Staining Buffer (Product # SB-4400) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 405 nm; Emission: 702 nm; Laser: Violet LaserBackground/Target InformationPDGFRA is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. Studies in knockout mice, where homozygosity is lethal, indicate that the alpha form of the platelet-derived growth factor receptor is particularly important for kidney development since mice heterozygous for the receptor exhibit defective kidney phenotypes.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
| 数据 |
| CD140a (PDGFRA) Antibody (67-1401-80) in FlowStaining of NIH/3T3 cells with 0.5 µg of Rat IgG2a K Isotype Control, Super Bright 702(Product # 67-4321-82) (blue histogram) or 0.5 µg of CD140a (PDGF Receptor a) MonoclonalAntibody, Super Bright 702 (purple histogram). Total viable cells were used for analysis |
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