Mouse IgG1 kappa Isotype Control, PE-Cyanine5

货号：15-4714-41,15-4714-42

规格：25 Tests,100 Tests

价格：0,1632

产品类型：小鼠同型对照

品牌：eBioscience

物种：其它

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：P3.6.2.8.1

荧光染料：PE-Cy5

抗体类型:	单抗	同型对照：
浓度：	5 µL/Test	用法：	Assay-Dependent(Ctrl)； 5 µL (0.25 µg)/test(Flow)

产品详细信息Description: The monoclonal mouse IgG1 K immunoglobulin is useful as an isotype control.Applications Reported: PE-Cy5 Mouse IgG1 K Isotype Control has been reported for use in flow cytometric analysis.Applications Tested: Thhis Mouse IgG1 K Isotype Control is offered in 2 formats: - µg size: This can be used at the same concentration as the experimental antibody. - test size: has been pre-titrated and tested by staining and flow cytometric analysis. This can be used at test size: 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.仅用于科研。不用于诊断过程。

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流式抗体

参考文献：

1.Scientific reportsPsoriasis patients demonstrate HLA-Cw*06:02 allele dosage-dependent T cell proliferation when treated with hair follicle-derived keratin 17 protein."15-4714 was used in Flow cytometry/Cell sorting to examine the Keratin 17 protein as the autoantigen in psoriasis, and the correlation between HLA-Cw*06:02 allele dosage and the T cell response."AuthorsYunusbaeva M,Valiev R,Bilalov F,Sultanova Z,Sharipova L,Yunusbayev B2.International journal of nanomedicineFunctionalization-dependent effects of cellulose nanofibrils on tolerogenic mechanisms of human dendritic cells."15-4714 was used in Flow cytometry/Cell sorting to investigate how differentially functionalised cellulose nanofibrils affect dendritic cell properties, their viability, morphology, differentiation and maturation potential, and the capacity to regulate T cell-mediated immune response."AuthorsTomić S,Ilić N,Kokol V,Gruden-Movsesijan A,Mihajlović D,Bekić M,Sofronić-Milosavljević L,Čolić M,Vučević D3. PloS oneSize-dependent effects of gold nanoparticles uptake on maturation and antitumor functions of human dendritic cells in vitro."15-4714 was used in Flow cytometry/Cell sorting to study the immunological effects of the internalisation of differently sized gold nanoparticles in a human dendritic cell model."AuthorsTomić S,Ðokić J,Vasilijić S,Ogrinc N,Rudolf R,Pelicon P,Vučević D,Milosavljević P,Janković S,Anžel I,Rajković J,Rupnik MS,Friedrich B,Colić M4.Journal of immunological methodsAn 11-color flow cytometric assay for identifying, phenotyping, and assessing endocytic ability of peripheral blood dendritic cell subsets in a single platform."15-4714 was used in Flow cytometry/Cell sorting to identify, pheontype, and assess endocytic ability of peripheral blood dendritic cell subsets in a single platform using an 11-color flow cytometric assay."AuthorsWang JC,Kobie JJ,Zhang L,Cochran M,Mosmann TR,Ritchlin CT,Quataert SA5.RetrovirologyB cells and monocytes from patients with active multiple sclerosis exhibit increased surface expression of both HERV-H Env and HERV-W Env, accompanied by increased seroreactivity."15-4714 was used in Flow cytometry/Cell sorting to analyse HERV-H Env and HERV-W Env epitope expression on the surface of PBMCs from MS patients with active and stable disease."AuthorsBrudek T,Christensen T,Aagaard L,Petersen T,Hansen HJ,Møller-Larsen A

技术参数

产品应用 Ctrl；Flow

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