Rat IgG2b kappa Isotype Control, Functional Grade

货号：16-4031-81,16-4031-85

规格：50 µg,500 µg

价格：717,2177

产品类型：大鼠同型对照

品牌：eBioscience

物种：其它

宿主：大鼠

抗体亚型：IgG2b, kappa

克隆号：eB149/10H5

荧光染料：其它

抗体类型:	同型对照	同型对照：	IgG2b, kappa
免疫原性：		用法：

Description: The rat IgG2b, kappa monoclonal antibody is useful as an isotype control immunoglobulin.Applications Reported: The monoclonal rat IgG2b kappa isotype control has been reported for use in flow cytometric analysis.Applications Tested: This rat IgG2b isotype control has been tested by flow cytometric analysis of mouse splenocyte suspensions. It can be used at the same concentration as the experimental antibody.Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.Background/Target InformationThe isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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参考文献：

1.PLoS pathogensDectin-2 is a primary receptor for NLRP3 inflammasome activation in dendritic cell response to Histoplasma capsulatum."16-4031 was used as a Control in experiments to investigate how H. capsulatum induces inflammasome activation and IL-1β production, showing that dectin-2 is a primary receptor for NLRP3 inflammasome activation."Authors Chang TH,Huang JH,Lin HC,Chen WY,Lee YH,Hsu LC,Netea MG,Ting JP,Wu-Hsieh BA2.Nature communicationsEarly ERK1/2 activation promotes DRP1-dependent mitochondrial fission necessary for cell reprogramming.Authors Prieto J,León M,Ponsoda X,Sendra R,Bort R,Ferrer-Lorente R,Raya A,López-García C,Torres J3.The Journal of neuroscience : the official journal of the Society for NeuroscienceDepletion of Ly6G/Gr-1 leukocytes after spinal cord injury in mice alters wound healing and worsens neurological outcome."16-4031 was used in Flow cytometry/Cell sorting to further the knowledge of the functions of neuroinflammation in spinal cord injury (SCI) in mice."Authors Stirling DP,Liu S,Kubes P,Yong VW4.Investigative ophthalmology and visual scienceDifferential effects of costimulatory pathway modulation on corneal allograft survival."16-4031 was used in Immunohistochemistry to examine the effect on corneal allograft survival of modulation of programmed death-1 and inducible costimulatory molecule."Authors Watson MP,George AJ,Larkin DF5.Cancer researchTumor-Promoting Effects of Myeloid-Derived Suppressor Cells Are Potentiated by Hypoxia-Induced Expression of miR-210."16-4031 was used as a Control in experiments to provide a preclinical rationale to explore miR-210 inhibitory oligonucleotides as adjuvants to boost immunotherapeutic responses in cancer patients."Authors Noman MZ,Janji B,Hu S,Wu JC,Martelli F,Bronte V,Chouaib S

技术参数

产品应用 Flow；FN；Neu；IHC

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