DyLight 594 Maleimide/DyLight 594马来酰亚胺
货号：46608
规格：1 mg
价格：4326
产品类型：蛋白标记
品牌：Thermo Fisher
| Thermo Scientific DyLight 594 Sulfhydryl-Reactive Dye is a maleimide-activated derivative of high-performance DyLight 594 used to fluorescently label sulfhydryl-containing peptides, proteins and other biomolecular probes.DyLight 594provides vibrant orange-to-red fluorescence with better performance than other rhodamine derivatives including Alexa Fluor 594 and Texas Red™ dye for fluorescent applications over a broad pH range (pH 4-9). The high water solubility of DyLight Fluors means that a high dye-to-protein ratio can be attained without causing precipitation of the conjugates.DyLight 594 Sulfhydryl-Reactive Dye is activated with a maleic acid imide (maleimide) moiety to form a reactive alkylation reagent. Labeling occurs through reaction of the maleimide-activated dye with reduced sulfhydryl groups (-SH) to form stable thioether bonds. Maleimides are specific for sulfhydryl groups between pH 6.5-7.5. Learn more aboutmaleimide chemistry. |
| 特点 |
| •High performance—DyLight 594 shows brighter fluorescence than Alexa Fluor 594 and Texas Red•Specific—maleimide-activated dye labels proteins and other molecules at reduced sulfhydryls (-SH)•Efficient labeling methods—well-characterized chemistry and optimized protocols provide for reliable, high-quality labeling•Optimized antibody labeling procedure—complete protocol for IgG reduction and labeling and calculating the labeling efficiency |
| 应用 |
| • Antibody labeling for immunofluorescence applications, including immunocytochemistry (ICC), immunohistochemistry (IHC), Western blotting and ELISA assay• Target macromolecule labeling for in vitro and in vivo fluorescent detection strategies |
| 数据 |
| Immunofluorescence microscopy using DyLight 594 dyeCytokeratin 18 (red) in A549 cells was fluorescently labeled using an anti-cytokeratin 18 primary antibody and DyLight 594-Conjugated Anti-Rabbit Secondary Antibody. Thermo Scientific Pierce Hoechst stain was used to fluorescently label cell nuclei. |
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