CD11b (activation epitope) Monoclonal Antibody (CBRM1/5), PE, eBioscience™

货号：12-0113-42

规格：100 Tests

价格：2721

产品类型：细胞生物学

品牌：eBioscience

抗体类型:	单抗	同型对照：	Mouse IgG1 kappa Isotype Control, PE, eBioscience™
浓度：	5 µL/Test	用法：	5 μL(0.25 μg)/test(Flow)

Description: The CBRM1/5 monoclonal antibody reacts with an activation-specific epitope of human Mac-1. CBRM1/5 binds a subset of Mac-1 molecules on neutrophils and monocytes after stimulation with chemoattractants or phorbol esters but does not recognize Mac-1 on resting myeloid cells. Through interactions with its ligands, Mac-1 participates in adhesive cell interactions. The epitope recognized by this mAb localizes to the I domain on the alpha chain of Mac-1 very close to the ligand binding site in a region that is widely exposed. CBRM1/5 blocks Mac-1 dependent adhesion to fibrinogen and ICAM-1 and inhibits chemoattractant-stimulated adhesion of eosinophils to the Intercellular Adhesion Molecule-1 (ICAM-1). It should be noted that low level activation may occur during processing of freshly drawn blood. Therefore the CBRM1.5 antibody may exhibit some binding to Mac-1 in these unstimulated samples. However, higher levels of Mac-1 expression are observed in activated samples when compared to unstimulated cells.Applications Reported: The CBRM1/5 antibody has been reported for use in flow cytometric analysis.Applications Tested: This CBRM1/5 antibody has been pre-titrated and tested by flow cytometric analysis of resting and activated normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationCD11b (integrin alpha-M, ITGAM, integrin alpha-X, ITGAX) is a 165 kDa adhesion molecule that associates non-covalently with integrin beta-2 (CD18). The CD11b/CD18 heterodimeric complex is also known as integrin alpha-M beta-2, Mac-1, and CR3 (complement receptor 3). CD11b is expressed on the surface of monocytes/macrophages, granulocytes, activated lymphocytes, a subset of NK cells, a subset of dendritic cells, and microglia in the brain. CD11b/CD18 functions as the receptor for ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X -these adhesions are critical for cell-cell and cell-matrix interactions. CD11b is expressed on 8% of spleen cells, 44% of bone marrow cells, and less than 1% of thymocytes, and is commonly used as a microglial marker in nervous tissue. The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Further, peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages. Diseases associated with CD11b dysfunction include systemic lupus erythematosus 6 and ITGAM-related susceptibility to systemic lupus erythematosus.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

CD11b (activation epitope) Antibody (12-0113-42) in FlowStaining of unstimulated (left) or 10-minute Manganese-stimulated (1 mM) (right) human peripheral blood cells withMouse IgG1 kappa Isotype Control PE (Product # 12-4714-81) (open histogram) or Anti-Human CD11b PE(filled histogram). Cells in the monocyte gate were used for analysis.

推荐产品：

二抗荧光二抗免疫组化一抗标签抗体

参考文献：

1. BioMed research internationalA Novel Murine Anti-Lactoferrin Monoclonal Antibody Activates Human Polymorphonuclear Leukocytes through Membrane-Bound Lactoferrin and TLR4."12-0113 was used in Flow cytometry/Cell sorting to investigate the function of membrane-bound lactoferrin in leukocytes."AuthorsHu XM,Xu YR,Yan R,Sun SL,Dong HL,Wang J,Gao XM2. Stem cells internationalA Member of the Nuclear Receptor Superfamily, Designated as NR2F2, Supports the Self-Renewal Capacity and Pluripotency of Human Bone Marrow-Derived Mesenchymal Stem Cells."12-0113 was used in Flow cytometry/Cell sorting to determine NR2F2 plays an important role in the stemness maintenance of bone marrow-derived mesenchymal stem cells."AuthorsZhu N,Wang H,Wang B,Wei J,Shan W,Feng J,Huang H3. Molecular medicine reportsNLS‑RARα modulates acute promyelocytic leukemia NB4 cell proliferation and differentiation via the PI3K/AKT pathway."12011342 was used in flow cytometry to explore the role of NLS-RARalpha in acute promyelocytic leukemia"AuthorsSong H,Li L,Zhong L,Yang R,Jiang K,Yang X,Liu B4. Kidney internationalComplement receptor 3 mediates renal protection in experimental C3 glomerulopathy."12-0113 was used in Flow cytometry/Cell sorting to explore the protective role of CR3 in glomerulonephritis and assess whether pharmacological potentiation of a CR3 interaction with iC3b could represent a novel therapeutic approach to C3 glomerulopathy."AuthorsBarbour TD,Ling GS,Ruseva MM,Fossati-Jimack L,Cook HT,Botto M,Pickering MC5. HaematologicaLenalidomide interferes with tumor-promoting properties of nurse-like cells in chronic lymphocytic leukemia."12-0113 was used in Flow cytometry/Cell sorting to investigate the effect of lenalidomide on the monocyte and macrophage populations in chronic lymphocytic leukemia patients."AuthorsFiorcari S,Martinelli S,Bulgarelli J,Audrito V,Zucchini P,Colaci E,Potenza L,Narni F,Luppi M,Deaglio S,Marasca R,Maffei R

技术参数

产品应用 Flow

© Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://biorod.cn/thread-16900.htm