CD3 Monoclonal Antibody (17A2), FITC, eBioscience™

货号：11-0032-80,11-0032-82

规格：25 µg,100 µg

价格：428,936

产品类型：流式抗体

品牌：eBioscience

抗原：

物种：小鼠

宿主：大鼠

抗体亚型：IgG2b, kappa

克隆号：17A2

荧光染料：FITC

抗体类型:	单抗	同型对照：	Rat IgG2b kappa Isotype Control (eB149/10H5), FITC, eBioscience™
浓度	0.5 mg/mL	用法：	0.5 µg/test(Flow);10 µg/mL(ICC)；10 µg/mL(IF)

产品详细信息

Description: The 17A2 monoclonal antibody reacts with the mouse CD3 complex. CD3 subunits gamma, delta and epsilon are required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Binding of 17A2 to CD3 initiates the intracellular biochemical pathway resulting in cellular activation and proliferation.Applications Reported: This 17A2 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 17A2 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. This 17A2 antibody has been tested by immunohistochemistry on frozen mouse spleen and can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

The CD3 subunit complex which is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules and this association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. The genes encoding the CD3 epsilon, gamma and delta polypeptides are located on chromosome 11. Defects in the CD3 gene are associated with CD3 immunodeficiency.

数据

CD3 Antibody (11-0032-80) in IFImmunohistochemistry of frozen mouse spleen stained with 10 µg/mL Anti-Mouse CD3 FITC and costained with 10 µg/mL Anti-Human/Mouse CD45R (B220) eFluor® 660. Nuclei are stained with DAPI. CD3 Antibody (11-0032-80) in FlowStaining of C57Bl/6 splenocytes with Anti-Human/Mouse CD45R (B220) PE (Product # 12-0452-82) and 0.125 µg of Rat IgG2b K Isotype Control FITC (Product # 11-4031-82) (left) or 0.125 µg of Anti-Mouse CD3 FITC (right). Total viable cells were used for analysis.

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参考文献：

1.PloS oneRottlerin as a therapeutic approach in psoriasis: Evidence from in vitro and in vivo studies.2.Frontiers in immunologyUpregulation of Intestinal Barrier Function in Mice with DSS-Induced Colitis by a Defined Bacterial Consortium Is Associated with Expansion of IL-17A Producing Gamma Delta T Cells.3.Genes and developmentPHF6 regulates phenotypic plasticity through chromatin organization within lineage-specific genes.4.Nature communications CD4+ T cells are activated in regional lymph nodes and migrate to skin to initiate lymphedema.5.International journal of molecular medicineSilybin attenuates LPS-induced lung injury in mice by inhibiting NF-κB signaling and NLRP3 activation.

技术参数

产品应用 Flow；IF；ICC；IF；IHC；ELISA

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