CD3e Monoclonal Antibody (eBio500A2 (500A2)), FITC, eBioscience™

货号：11-0033-82

规格：100 µg

价格：1046

产品类型：流式抗体

品牌：eBioscience

抗原：

物种：小鼠

宿主：其它

抗体亚型：IgG

克隆号：eBio500A2 (500A2)

荧光染料：FITC

抗体类型:	流式抗体	同型对照：	Syrian Hamster IgG Isotype Control, FITC, eBioscience™
浓度：	0.5 mg/mL	用法：	0.5 µg/test(Flow)

产品详细信息Description: The eBio500A2 monoclonal antibody reacts with the 25 kD epsilon subunit of the mouse CD3 complex. CD3 subunits gamma, delta and epsilon are required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells, as well as NKT cells. Binding of eBio500A2 to CD3 initiates the intracellular biochemical pathway resulting in cellular activation and proliferation. The 500A2 antibody is able to partially cross-block the 17A2 and 145-2C11 antibodies, indicating that all three of these anti-CD3 antibodies recognize distinct but overlapping epitopes.Applications Reported: This eBio500A2 (500A2) antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBio500A2 (500A2) antibody has been tested by flow cytometric analysis. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered. 靶标信息The CD3 subunit complex which is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules and this association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. The genes encoding the CD3 epsilon, gamma and delta polypeptides are located on chromosome 11. Defects in the CD3 gene are associated with CD3 immunodeficiency.

数据

CD3e Antibody (11-0033-81) in Flow Staining of BALB/c splenocytes with Anti-Mouse CD19 APC (Product # 17-0191-82) and 0.25 µg of Golden Syrian Hamster IgG Isotype Control FITC (Product # 11-4914-81) (left) or 0.25 µg of Anti-Mouse CD3e FITC (right). Cells in the lymphocyte gate were used for analysis. CD3e Antibody (11-0033-81)Journal of the American Society of Nephrology : JASN 2011 -Published figure using CD3e monoclonal antibody (Product # 11-0033-81)

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参考文献：

1.Scientific reports Activation of the Renin-Angiotensin System Promotes Colitis Development.2.Cardiovascular research Leucocyte expression of complement C5a receptors exacerbates infarct size after myocardial reperfusion injury.3.Biological research Immune enhancement activities of silk lutein extract from Bombyx mori cocoons.4.Autophagy Reciprocal effects of rab7 deletion in activated and neglected T cells.5.Journal of immunology (Baltimore, Md. : 1950) Staphylococcus aureus induces type I IFN signaling in dendritic cells via TLR9.

技术参数

产品应用 Flow；IHC

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