CD4 Monoclonal Antibody (OX35), FITC, eBioscience™

货号：11-0040-81,11-0040-82,11-0040-85

规格：50 µg,100 µg,500 µg

价格：750,1201,2771

产品类型：流式抗体

品牌：eBioscience

抗原：CD4

物种：大鼠

宿主：小鼠

抗体亚型：其它

克隆号：OX35

荧光染料：FITC

抗体类型:	流式抗体	同型对照：	Mouse IgG2a kappa Isotype Control (eBM2a), FITC, eBioscience™
浓度：	0.5 mg/mL	用法：	0.25 µg/test (Flow）

产品详细信息Description: The OX35 monoclonal antibody reacts with rat CD4. CD4 is a co-receptor which participates in signaling through the T cell receptor by making contacts with MHC class II expressed on antigen-presenting cells (APC). CD4 is expressed in the thymus by CD4+CD8+ "double-positive" and CD4+ "single-positive" thymocytes, and by CD4+ "helper" T cells in the periphery. CD4 is a cell-surface membrane glycoprotein which contains four extracellular immunoglobulin-like domains.Applications Reported: This OX35 antibody has been reported for use in flow cytometric analysis.Applications Tested: This OX35 antibody has been tested by flow cytometric analysis of rat splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered. 靶标信息The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017].

数据

CD4 Antibody (11-0040-81) in FlowStaining of rat splenocytes with Anti-Rat CD3 PE (Product # 12-0030-82) and 0.125 µg of Mouse IgG2a K Isotype Control FITC (Product # 11-4724-42) (left) or 0.125 µg of Anti-Rat CD4 FITC (right). Cells in the lymphocyte gate were used for analysis.CD4 Antibody (11-0040-81)Molecular medicine reports 2017 -Figure 2. Expression of IL-17 and CD4 in the ipsilateral dorsal horn of L5-6 spinal cord following SNL, and IL-17 in the serum. (A) Quantitative polymerase chain reaction analysis of IL-17 mRNA expression in the rat spinal dorsal horn over time following SNL compared with sham-operated and naive animals, respectively. Data are expressed as mean fold +- SEM (n=4/group). *P<0.05 vs. the sham-operated rats at day 7 post-surgery, as determined by two-way analysis of variance, followed by Bonferroni and apos;s post-hoc test. (B) Serum IL-17 measured by ELISA at days 7 and 14 post-surgery. Data are expressed as the mean +- SEM (n=6/group). (C) Double immunofluorescence staining with antibodies against IL-17 and CD4 in the ipsilateral L5 spinal dorsal horn at day 7 following SNL compared with the sham group. The representative images from at least three independent experiments depicted the IL-17A/CD4 overlay, indicating colocalization of IL-17A immunoreactivity with CD4 + cells. The images are representative images of at least three independent experiments. (D) CD4 + T cell infiltration into the rats and apos; ipsilateral lumbar spinal cord at L5-L6 at days 7 and 14 post-SNL, the sham operation or naive rats via FACS. Lumbar spinal cord mononuclear cells were isolated and labeled with anti-rat CD3-FITC and CD4-PE monoclonal antibodies at days 7 and 14, and then were analyzed in CD3 vs. CD4 dot plots. The images are representative of three independent experiments at day 7 and 14, respectivel

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参考文献：

1.Molecular medicine reportsBiological effects of bone marrow mesenchymal stem cells on hepatitis B virus invitro.2.Mediators of inflammationDeficits in endogenous adenosine formation by ecto-5'-nucleotidase/CD73 impair neuromuscular transmission and immune competence in experimental autoimmune myasthenia gravis.3.PloS oneThe mechanism of effective electroacupuncture on T cell response in rats with experimental autoimmune encephalomyelitis.4.International journal of rheumatologyChanges of CD4(+) CD25(+) Regulatory T Cells, FoxP3 in Adjuvant Arthritis Rats with Damage of Pulmonary Function and Effects of Tripterygium Glycosides Tablet.5.Journal of cellular and molecular medicineImmunogenicity of allogeneic mesenchymal stem cells.

技术参数

产品应用 Flow；IF

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