Rat IgG2a kappa Isotype Control, FITC

货号：11-4321-80,11-4321-81,11-4321-82,11-4321-85

规格：25 µg ,50 µg ,100 µg ,500 µg

价格：527,851,1383,2127

产品类型：大鼠同型对照

品牌：eBioscience

物种：其它

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：eBR2a

荧光染料：FITC

抗体类型:	同型对照	同型对照：
浓度：	0.5 mg/mL	用法：	Assay-Dependent(IHC)；Assay-Dependent(ICC)；Assay-Dependent(IF)；Assay-Dependent(Flow)；Assay-Dependent(Ctrl)

Description: The monoclonal rat IgG2a, kappa is useful as an isotype control immunoglobulin.Applications Reported: This rat IgG2a isotype control has been reported for use in immunocytochemistry, immunohistochemistry, and flow cytometric analysis.Applications Tested: This rat IgG2a isotype control has been tested by flow cytometric analysis of mouse splenocytes. This can be used at the same concentration as the experimental antibody.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationThe isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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参考文献：

1.Molecular therapy oncolyticsIntratumoral INF-γ triggers an antiviral state in GL261 tumor cells: a major hurdle to overcome for oncolytic vaccinia virus therapy of cancer."11-4321 was used in Flow cytometry/Cell sorting to identify microenvironmental factors, which may be decisive for failure or success of oncolytic VACV therapy."AuthorsKober C,Weibel S,Rohn S,Kirscher L,Szalay AA 2.Experimental and therapeutic medicineEffect of cold stress on immunity in rats."11-4321 was used in Flow cytometry/Cell sorting to determine the effect of a sudden ambient temperature drop on the levels of stress hormones and T-lymphocyte cytokines in the plasma of mice."AuthorsHu GZ,Yang SJ,Hu WX,Wen Z,He D,Zeng LF,Xiang Q,Wu XM,Zhou WY,Zhu QX 3.Molecular and cellular biologyImmunosuppression by N-methyl-D-aspartate receptor antagonists is mediated through inhibition of Kv1.3 and KCa3.1 channels in T cells."11-4321 was used in Flow cytometry/Cell sorting to investigate the effect of NMDAR antagonists on T-cell function, showing that antagonists cause immunosuppression via inhibition of Kv1.3 and KCa3.1 channels in T-cells."AuthorsKahlfuß S,Simma N,Mankiewicz J,Bose T,Lowinus T,Klein-Hessling S,Sprengel R,Schraven B,Heine M,Bommhardt U 4.BioMed research internationalCharacterization of cardiac-resident progenitor cells expressing high aldehyde dehydrogenase activity."11-4321 was used in Flow cytometry/Cell sorting to investigate the presence of cardiac-resident progenitor cells with high aldehyde dehydrogenase activity isolated from mouse hearts."AuthorsRoehrich ME,Spicher A,Milano G,Vassalli G 5.VirologyRole of regulatory T cells in coronavirus-induced acute encephalitis."11-4321 was used in Flow cytometry/Cell sorting to show that Tregs may help to limit immunopathological disease without delaying virus clearance in coronavirus-induced acute encephalitis."AuthorsAnghelina D,Zhao J,Trandem K,Perlman S

技术参数

产品应用 Flow；IF；ICC；IHC

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