CD3e Monoclonal Antibody (eBio500A2 (500A2)), Functional Grade, eBioscience™/CD3e单抗 (eBio500A2 (500A2)), 功能抗体, eBioscience™

货号：16-0033-81,16-0033-82,16-0033-85,16-0033-86,16-0033-38,16-0033-025,16-0033-050

规格：50 µg,100 µg,500 µg,1 mg,5mg,25 mg,50 mg

价格：934,1369,2636,4818,24089,72266,120443

产品类型：流式抗体

品牌：eBioscience

抗原：CD3e

物种：小鼠

宿主：其它

抗体亚型：其它

克隆号：eBio500A2 (500A2)

荧光染料：其它

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类型:	一抗(功能抗体）	同型对照：	Syrian Hamster IgG Isotype Control, Functional Grade, eBioscience™
浓度：	1 mg/mL	用法：	0.5 µg/test（Flow）

产品详细信息Description: The eBio500A2 monoclonal antibody reacts with the 25 kD epsilon subunit of the mouse CD3 complex. CD3 subunits gamma, delta and epsilon are required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells, as well as NKT cells. Binding of eBio500A2 to CD3 initiates the intracellular biochemical pathway resulting in cellular activation and proliferation. The 500A2 antibody is able to partially cross-block the 17A2 and 145-2C11 antibodies, indicating that all three of these anti-CD3 antibodies recognize distinct but overlapping epitopes.Applications Tested: This eBio500A2 (500A2) antibody has been tested by flow cytometric analysis of mouse thymocytes and splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息The CD3 subunit complex which is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components havelong cytoplasmic tails that associate with cytoplasmic signal transduction molecules and this association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. The genes encoding the CD3 epsilon, gamma and delta polypeptides are located on chromosome 11. Defects in the CD3 gene are associated with CD3 immunodeficiency.仅用于科研。不用于诊断过程。未经明确授权不得转售。

数据

CD3e Antibody (16-0033-82) in FlowStaining of BALB/c splenocytes with Anti-Mouse CD19 APC (Product # 17-0193-82) and 0.25 µg of Golden Syrian hamster IgG Isotype Control Functional Grade Purified (Product # 16-4914-85) (left) or 0.25 µg of Anti-Mouse CD3e Functional Grade Purified (right) followed by Anti-Syrian Hamster IgG FITC (Product # 11-4211-85).Cells in the lymphocyte gate were used for analysis.

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参考文献：

1.Human vaccines and immunotherapeuticsImmunogenicity of a novel enhanced consensus DNA vaccine encoding the leptospiral protein LipL45."16-0033 was used in Flow cytometry/Cell sorting to test the efficacy of a synthetic consensus DNA vaccine developed against the Leptospira membrane lipoprotein LipL45, demonstrating that this LipL45 based DNA immunogen has potential as a anti-Leptospira vaccine."AuthorsVijayachari P,Vedhagiri K,Mallilankaraman K,Mathur PP,Sardesai NY,Weiner DB,Ugen KE,Muthumani K2.Scientific reportsLatent virus infection upregulates CD40 expression facilitating enhanced autoimmunity in a model of multiple sclerosis."16-0033 was used in Flow cytometry/Cell sorting to investigate the cause of enhanced experimental autoimmune encephalomyelitis following infection with murine γ-herpesvirus 68."AuthorsCasiraghi C,Márquez AC,Shanina I,Horwitz MS3.Nature communicationsDiet and specific microbial exposure trigger features of environmental enteropathy in a novel murine model."16-0033 was used in Flow cytometry/Cell sorting to provide evidence indicating that both diet and microbes combine to contribute to the aetiology of environmental enteropathy."AuthorsBrown EM,Wlodarska M,Willing BP,Vonaesch P,Han J,Reynolds LA,Arrieta MC,Uhrig M,Scholz R,Partida O,Borchers CH,Sansonetti PJ,Finlay BB4.Nature communicationsA mobile endocytic network connects clathrin-independent receptor endocytosis to recycling and promotes T cell activation."16-0033 was used in Functional assays to examine the endocytic journey of the T cell receptor."AuthorsCompeer EB,Kraus F,Ecker M,Redpath G,Amiezer M,Rother N,Nicovich PR,Kapoor-Kaushik N,Deng Q,Samson GPB,Yang Z,Lou J,Carnell M,Vartoukian H,Gaus K,Rossy J5.PloS one Effective chemoimmunotherapy with anti-TGFβ antibody and cyclophosphamide in a mouse model of breast cancer. "16-0033 was used in Functional assays to investigate whether TGFβ is responsible for the accumulation of Tregs in tumours." AuthorsChen X,Yang Y,Zhou Q,Weiss JM,Howard OZ,McPherson JM,Wakefield LM,Oppenheim JJ

技术参数

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