CD14 Monoclonal Antibody (61D3), APC-eFluor 780

货号：47-0149-42,47-0149-41

规格：100 Tests,25 Tests

价格：3026,1316

产品类型：流式抗体

品牌：Thermo Fisher

物种：人

宿主：小鼠

抗体亚型：IgG

克隆号：61D3

荧光染料：APC-eFluor 780

抗体类型:	单抗	同型对照：	Mouse IgG1 kappa Isotype Control, APC-eFluor 780, eBioscience™
免疫原性：		用法：	5uL（0.5µg）/test(Flow）

Description: The 61D3 monoclonal antibody reacts with human CD14, a 53-55 kDa GPI-linked glycoprotein. CD14 is expressed on monocytes, interfollicular macrophages and some dendritic cells. Complexes of LPS and LBP (LPS-Binding Protein) bind with high affinity to monocytes through the surface CD14.Applications Reported: This 61D3 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 61D3 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. APC-eFluor® 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochrome.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationCD14 is a 55 kDa GPI-anchored glycoprotein that is constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils. CD14 also serves as a multifunctional lipopolysaccharide receptor, and is released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein. The soluble sCD14 can discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles. Further, CD14 has been shown to bind apoptotic cells, and can affect allergic, inflammatory and infectious processes. Alternative splicing results in multiple transcript variants encoding the same CD14 isoform. Diseases associated with CD14 dysfunction include mycobacterium chelonae infection and Croup.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

CD14 Antibody (47-0149-42) in Flow Staining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control APC-eFluor® 780 (Product # 47-4714-82) (blue histogram) or Anti-Human CD14 APC-eFluor® 780 (purple histogram). Cells in the monocyte gate were used for analysis.

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参考文献：

1. Stem cell research and therapyHuman umbilical cord mesenchymal stem cells improve the reserve function of perimenopausal ovary via a paracrine mechanism.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer2. The Journal of clinical investigationA therapeutic T cell receptor mimic antibody targets tumor-associated PRAME peptide/HLA-I antigens.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer3. Scientific reportsThrombomodulin regulates monocye differentiation via PKCδ and ERK1/2 pathway in vitro and in atherosclerotic artery.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer4. PloS oneInvestigating the causes for decreased levels of glutathione in individuals with type II diabetes.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer5. Journal of interferon and cytokine research : the official journal of the International Society for Interferon and Cytokine ResearchLiposomal Glutathione Supplementation Restores TH1 Cytokine Response to Mycobacterium tuberculosis Infection in HIV-Infected IndividualsBy clicking the link above, you will be redirected to the Benchsci website.Disclaimer

技术参数

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