CD11b Monoclonal Antibody (M1/70), APC-eFluor 780

货号： 47-0112-80, 47-0112-82

规格：25μg,100μg

价格：925,2027

产品类型：流式抗体

品牌：Thermo Fisher

物种：小鼠

宿主：大鼠

抗体亚型：IgG2b, kappa

克隆号：M1/70

荧光染料：APC-eFluor 780

抗体类型:	单抗	同型对照：	Rat IgG2b kappa Isotype Control, APC-eFluor 780, eBioscience™
免疫原性：		用法：	0.125 µg/test(Flow）

Description: The M1/70 monoclonal antibody reacts with mouse CD11b, the 165-170 kDa integrin alphaM. CD11b non-covalently associates with CD18 to form alphaMbeta2 integrin (Mac-1) and binds to CD54 (ICAM-1), C3bi, and fibrinogen. Mac-1 is expressed by macrophages, NK cells, granulocytes, activated lymphocytes and mouse B-1 cells in the peritoneal cavity. M1/70 is also cross-reactive to human CD11b, and can be used for the detection of this antigen on human peripheral blood monocytes, granulocytes, and a subset of NK cells. Through interactions with its ligands, CD11b participates in adhesive cell interactions.Applications Reported: This M1/70 antibody has been reported for use in flow cytometric analysis.Applications Tested: This M1/70 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Light sensitivity: This tandem is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationCD11b (integrin alpha-M, ITGAM, integrin alpha-X, ITGAX) is a 165 kDa adhesion molecule that associates non-covalently with integrin beta-2 (CD18). The CD11b/CD18 heterodimeric complex is also known as integrin alpha-M beta-2, Mac-1, and CR3 (complement receptor 3). CD11b is expressed on the surface of monocytes/macrophages, granulocytes, activated lymphocytes, a subset of NK cells, a subset of dendritic cells, and microglia in the brain. CD11b/CD18 functions as the receptor for ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X -these adhesions are critical for cell-cell and cell-matrix interactions. CD11b is expressed on 8% of spleen cells, 44% of bone marrow cells, and less than 1% of thymocytes, and is commonly used as a microglial marker in nervous tissue. The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Further, peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages. Diseases associated with CD11b dysfunction include systemic lupus erythematosus 6 and ITGAM-related susceptibility to systemic lupus erythematosus.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

CD11b Antibody (47-0112-80) in Flow Staining of C57BL/6 bone marrow cells with 0.06 µg of Rat IgG2b kappa Isotype Control APC-eFluor® 780 (Product # 47-4031-82) (blue histogram) or 0.06 µg of Anti-Mouse CD11b APC-eFluor® 780 (purple histogram). Total viable cells were used for analysis.

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参考文献：

1. Nature medicineJNK1 negatively controls antifungal innate immunity by suppressing CD23 expression.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer2. The Journal of clinical investigationA CCR4 antagonist reverses the tumor-promoting microenvironment of renal cancer.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer3. NatureMyeloid progenitor cluster formation drives emergency and leukaemic myelopoiesis.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer4. RNA biologyADAR1 deletion induces NFκB and interferon signaling dependent liver inflammation and fibrosis.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer5. Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and MetabolismDepletion of microglia exacerbates postischemic inflammation and brain injury.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer

技术参数

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