CD8a Monoclonal Antibody (53-6.7), APC-eFluor 780

货号：47-0081-80,47-0081-82

规格：50μg,100μg

价格：925,2013

产品类型：流式抗体

品牌：Thermo Fisher

物种：小鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：53-6.7

荧光染料：APC-eFluor 780

抗体类型:	单抗	同型对照：	Rat IgG2a kappa Isotype Control, APC-eFluor 780, eBioscience™
免疫原性：		用法：	0.5 µg/test(Flow）

Description: The 53-6.7 monoclonal antibody reacts with the mouse CD8a molecule. CD8a is an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha beta) or as a homodimer (CD8 alpha alpha). A majority of thymocytes and a subpopulation of mature alpha beta TCR T cells express CD8 alpha beta while gamma delta TCR T cells, a subpopulation of intestinal intraepithelial lymphocytes (IELs) and dendritic cells express CD8 alpha alpha. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells.Applications Reported: This 53-6.7 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 53-6.7 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Light sensitivity: This tandem is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationCD8 (Cluster of Differentiation 8) is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediate efficient cell-cell interactions within the immune system. The CD8 antigen acts as a co-receptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. CD8 is found on a T cell subset of normal cytotoxic/suppressor cells which make up approximately 20-35 % of human peripheral blood lymphocytes. The CD8 antigen is also detected on natural killer (NK) cells, subpopulations of peripheral blood null cells, thymocytes and bone marrow cells. The CD8 co-receptor functions as either a homodimer composed of two alpha chains, or as a disulfide-linked heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. The majority of CD8+ T cells express CD8 as a alpha/beta heterodimer. In HIV, the HIV-2 envelope glycoprotein binds CD8 alpha chain (but not the beta chain).For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

CD8a Antibody (47-0081-82) in Flow Staining of BALB/c splenocytes with Anti-Mouse CD4 FITC (Product # 11-0041-82) and 0.25 µg of Rat IgG2a K Isotype Control APC-eFluor® 780 (Product # 47-4321-82) (left) or 0.25 µg of Anti-Mouse CD8a APC-eFluor® 780 (right).Total viable cells were used for analysis.

推荐产品：

细胞周期检测细胞凋亡检测细胞增殖活性检测

参考文献：

1. RNA biologyADAR1 deletion induces NFκB and interferon signaling dependent liver inflammation and fibrosis.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer2. OncoimmunologyIdentification of inhibitors of myeloid-derived suppressor cells activity through phenotypic chemical screening.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer3. Frontiers in immunologyT Cells.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer4. Immunity, inflammation and diseaseLocation of tumor affects local and distant immune cell type and number.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer5. The Journal of experimental medicineEgr2 and 3 control adaptive immune responses by temporally uncoupling expansion from T cell differentiation.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer

技术参数

产品应用 Flow

© Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://biorod.cn/thread-16991.htm