| 产品介绍 | | |
| Rat anti Mouse CD44 antibody, clone IM7recognizes CD44, also known as H-CAM, HUTCH and lymphocyte antigen 24 (Ly-24).CD44 is a cell surface receptor for hyaluronic acid, although it can also interact with other ligands such as collagens and matrix metalloproteinases. This protein plays a role in a variety of cellular functions, such as adhesion, lymphocyte activation and lymph node homing. It is also involved in cell migration and plays a role in tumor metastasis. CD44 has many distinct isoforms responsible for its functional diversity.Rat anti Mouse CD44 antibody, clone IM7 recognizes all isoforms of CD44 and has been reported to inhibit a delayed-type hypersensitivity responsein vivoand to induce complement-mediated cytotoxicity. |
| 产品详情 | | |
| Target Species | Mouse |
| Product Form | Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid |
| Preparation | Purified IgG prepared by affinity chromatography |
| Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | <0.1% Sodium Azide (NaN3) |
| Immunogen | Dexamethasone-induced myeloid leukemia M1 cells. |
| Approx. Protein Concentrations | IgG concentration 0.5 mg/ml |
| Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
| FITC | 490 | 525 |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| Store at +4℃. DO NOT FREEZE.This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | 1/20 | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 文献 |
| 1.Mckallip, R.J.et al.(2002) Role of CD44 in activation-induced cell death: CD44-deficient mice exhibit enhanced T cell response to conventional and superantigens.Int Immunol. 14 (9): 1015-26.2.Camp, R.L.et al.(1993) CD44 is necessary for optimal contact allergic responses but is not required for normal leukocyte extravasation.J Exp Med. 178: 497-507.3.Brocke, S.et al.(1999) Antibodies to CD44 and integrin alpha4, but not L-selectin, prevent central nervous system inflammation and experimental encephalomyelitis by blocking secondary leukocyte recruitment.Proc Natl Acad Sci U S A. 96: 6896-901.4.Lesley, J.et al.(1992) Requirements for hyaluronic acid binding by CD44: a role for the cytoplasmic domain and activation by antibody.J Exp Med. 175: 257-66. |
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