CD16/CD32 Monoclonal Antibody (93), PerCP-Cyanine5.5
货号:45-0161-80,45-0161-82
规格:25μg,100μg
价格:1363,3107
产品类型:流式抗体
品牌:Thermo Fisher
物种:小鼠
宿主:大鼠
抗体亚型:IgG2a Lamda
克隆号:93
荧光染料:PE-Cy5.5
抗体类型: | 单抗 | 同型对照: | IgG2a, kappa |
免疫原性: | | 用法: | 0.125 µg/test(Flow) |
Description: The 93 monoclonal antibody reacts with an epitope shared by mouse CD16 and CD32. CD16 (Fc gamma III Receptor) and CD32 (Fc gamma II Receptor) are the low affinity receptors for the mouse IgG Fc portion and are expressed by B cells, monocyte/macrophages, NK cells, and neutrophils.Applications Reported: This 93 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 93 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 695 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationCD16 is a 50-65 kDa cell surface molecule that exists in two forms - a transmembranous form expressed by natural killer (NK) cells, and some T cells and a phosphatidylinositol linked form expressed by granulocytes. CD16 is a low affinity receptor for IgG (FcR III), and is an important receptor mediating ADCC (antibody dependent cell mediated cytotoxicity) by NK cells. IN comparison, CD32 is a 40 kD glycoprotein that acts as a low affinity receptor for IgG (also known as Fc gamma RII), and mediates several functions including endocytosis, activation of secretion, cytotoxicity and immunomodulation. CD32 is expressed by B cells, monocytes, granulocytes and platelets. Further, CD32 is involved in the phagocytosis of immune complexes, the regulation of antibody production by B-cells, and variations of the CD32 gene may increase vulnerability to systemic lupus erythematosus (SLE). Several transcript variants encoding different isoforms of CD32 have been found.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
数据 |
CD16/CD32 Antibody (45-0161-80) in Flow Staining of BALB/c splenocytes with 0.06 µg of Rat IgG2a K Isotype Control PerCP-Cyanine5-5 (Product # 45-4321-80) (open histogram) or 0.06 µg of Anti-Mouse CD16/CD32 PerCP-Cyanine5-5 (filled histogram). Total viable cells were used for analysis. |
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参考文献: |
1. Nature communicationsRational combination of oncolytic vaccinia virus and PD-L1 blockade works synergistically to enhance therapeutic efficacy.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer2. NatureMyeloid progenitor cluster formation drives emergency and leukaemic myelopoiesis.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer3. PloS oneEndothelial Cell-Selective Adhesion Molecule Expression in Hematopoietic Stem/Progenitor Cells Is Essential for Erythropoiesis Recovery after Bone Marrow Injury."45-0161 was used in Flow cytometry/Cell sorting to determine the physiological importance of Endothelial Cell-Selective Adhesion Molecule (ESAM) in erythroid recovery, showing that ESAM is essential for erthryopoiesis recovery following bone marrow injury."AuthorsSudo T,Yokota T,Okuzaki D,Ueda T,Ichii M,Ishibashi T,Isono T,Habuchi Y,Oritani K,Kanakura Y4. Nature medicineThe creatine kinase pathway is a metabolic vulnerability in EVI1-positive acute myeloid leukemia."45-0161 was used in Flow cytometry/Cell sorting to investigate the effect of MECOM on cellular metabolism."AuthorsFenouille N,Bassil CF,Ben-Sahra I,Benajiba L,Alexe G,Ramos A,Pikman Y,Conway AS,Burgess MR,Li Q,Luciano F,Auberger P,Galinsky I,DeAngelo DJ,Stone RM,Zhang Y,Perkins AS,Shannon K,Hemann MT,Puissant A,Stegmaier K5. Journal for immunotherapy of cancerAgonist anti-GITR monoclonal antibody and stereotactic radiation induce immune-mediated survival advantage in murine intracranial glioma....By clicking the link above, you will be redirected to the Benchsci website.Disclaimer |
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