| 产品介绍 | | |
| Mouse anti Human CD7 antibody, clone LT7recognizes the human T-cell antigen CD7, also known as TP41 or CD7. CD7 is a single chain cell surface glycoprotein of ~40 kDa, expressed by T cells, NK cells and thymocytes. CD7 is also expressed by tumor cells in some cases of acute myeloid leukaemia. |
| 产品详情 | | |
Target Species | Human |
Product Form | Purified IgG - liquid |
Preparation | Purified IgG prepared by affinity chromatography on Protein A |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | 0.09% Sodium Azide |
Immunogen | Normal human peripheral blood lymphocytes. |
| Approx. Protein Concentrations | IgG concentration 1.0 mg/ml |
Fusion Partners | Spleen cells from immunized BALB/c mice were fused with cells of the X63.653 mouse myeloma cell line. |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
Flow Cytometry | √ | 1/50 | 1/100 |
Immunohistology - Frozen | √ | | |
Immunoprecipitation | √ | | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.Use 10ul of the suggested working dilution to label 106cells in 100ul |
| 同型对照 |
Description | Product Code | Applications | Pack Size |
| Mouse IgG2a NegativeControl | MCA929 | F | 100 Tests |
| 数据 |
| Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD7 antibody, clone CT7 (MCA1197). Low power.Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD7 antibody, clone CT7 (MCA1197). High power. |
| 文献 |
| 1. Haynes, B.F.et al.(1989) Ontogeny of T-cell precursors: a model for the initial stages of human T-cell development.Immunol Today. 10 (3): 87-91.2. Aruffo, A. & Seed, B. (1987) Molecular cloning of two CD7 (T-cell leukemia antigen) cDNAs by a COS cell expression system.EMBO J. 6 (11): 3313-6. |
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