CD19 Monoclonal Antibody (eBio1D3 (1D3)), PE-Cy7

货号：25-0193-82,25-0193-81

规格：100μg,50μg

价格：2019,1038

产品类型：流式抗体

品牌：Thermo Fisher

物种：小鼠

宿主：大鼠

抗体亚型：IgG

克隆号：eBio1D3 (1D3)

荧光染料：PE-Cy7

抗体类型:	单抗	同型对照：	Rat IgG2a kappa Isotype Control, PE-Cyanine7, eBioscience™
免疫原性：		用法：	0.25µg/test(Flow）

Description: The eBio1D3 monoclonal antibody reacts with mouse CD19, a 95 kDa transmembrane glycoprotein. CD19 is expressed by B cells during all stages of development excluding the terminally differentiated plasma cells. Follicular dendritic cells also express CD19. Together CD21, CD81, MHC class II, and CD19 form a multimolecular complex that associates with the BCR. Signaling through CD19 induces tyrosine phosphorylation, calcium flux and proliferation of B cells.Applications Reported: This eBio1D3 (1D3) antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBio1D3 (1D3) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationCD19 is a member of the immunoglobulin superfamily and has two Ig like domains. The CD19 molecule is expressed on 100% of the peripheral B cells as defined by expression of kappa or lambda light chains. CD19 appears to be expressed on myeloid leukemia cells, particularly those of monocytic lineage. Leukemia phenotype studies have demonstrated that the earliest and broadest B cell restricted antigen is the CD19 antigen. The receptor for CD19 is an important functional regulator of normal and malignant B cell proliferation, and is expressed in all B cell precursor leukemias. Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. CD19 is a cell surface molecule which assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. Besides being a signal-amplifying coreceptor for the B cell receptor (BCR), CD19 can also signal independently of BCR co-ligation and is a central regulatory component upon which multiple signaling pathways converge. Mutation of the CD19 gene results in hypogammaglobulinemia, whereas CD19 overexpression causes B cell hyperactivity.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

CD19 Antibody (25-0193-82) in Flow Staining of BALB/c splenocytes with Anti-Human/Mouse CD45R (B220) FITC (Product # 11-0452-82) and 0.125 µg of Rat IgG2a K Isotype Control PE-Cyanine7 (Product # 25-4321-82) (left) or 0.125 µg of Anti-Mouse CD19 PE-Cyanine7 (right). Cells in the lymphocyte gate were used for analysis.

推荐产品：

流式抗体 细胞周期检测 细胞凋亡检测 细胞增殖活性检测

参考文献：

1. RNA biologyADAR1 deletion induces NFκB and interferon signaling dependent liver inflammation and fibrosis.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer2. The Journal of clinical investigationA therapeutic T cell receptor mimic antibody targets tumor-associated PRAME peptide/HLA-I antigens.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer3. Nature communicationsT-B interactions are sufficient for inducing autoimmune-like chronic graft-versus-host disease.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer4. Nature communicationsNFAT2 is a critical regulator of the anergic phenotype in chronic lymphocytic leukaemia."25-0193 was used in Flow cytometry/Cell sorting to identify NFAT2 as a crucial regulator of the anergic phenotype in chronic lymphocytic leukaemia."AuthorsMärklin M,Heitmann JS,Fuchs AR,Truckenmüller FM,Gutknecht M,Bugl S,Saur SJ,Lazarus J,Kohlhofer U,Quintanilla-Martinez L,Rammensee HG,Salih HR,Kopp HG,Haap M,Kirschniak A,Kanz L,Rao A,Wirths S,Müller MR5. Cell reportsMononuclear Phagocytes in Cytokine-Induced Tissue Repair."25-0193 was used in Flow cytometry/Cell sorting to study the distinct kinase-independent role of RIPK3 in cytokine-induced tissue repair."AuthorsMoriwaki K,Balaji S,Bertin J,Gough PJ,Chan FK

技术参数

产品应用 Flow

© Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://biorod.cn/thread-17029.htm