Rat IgG1 kappa Isotype Control, APC

货号：17-4301-82,17-4301-81,17-4301-83

规格：100μg,50μg,200 μg

价格：2386,1632,3521

产品类型：大鼠同型对照

品牌：eBioscience

物种：其它

宿主：大鼠

抗体亚型：IgG1, kappa

克隆号：eBRG1

荧光染料：APC

抗体类型:	单抗	同型对照：
免疫原性：		用法：	Assay-Dependent (Ctrl)；Assay-Dependent (Flow)

Description: The monoclonal rat IgG1, kappa is useful as an isotype control immunoglobulin.Applications Reported: This rat IgG1 isotype control has been reported for use in flow cytometric analysis and ELISA.Applications Tested: This rat IgG1 isotype control has been used by flow cytometric analysis of mouse splenocyte suspension, This can be used at the same concentration as the experimental antibody.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationThe isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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参考文献：

1. OncotargetKITENIN promotes glioma invasiveness and progression, associated with the induction of EMT and stemness markers."17-4301 was used in Flow cytometry/Cell sorting to show that high KITENIN levels increases glioma invasiveness and progression, associated with up-regulation of EMT and stemness markers."AuthorsLee KH,Ahn EJ,Oh SJ,Kim O,Joo YE,Bae JA,Yoon S,Ryu HH,Jung S,Kim KK,Lee JH,Moon KS2. Infection and immunityEfficient B cell responses to Borrelia hermsii infection depend on BAFF and BAFFR but not TACI."17-4301 was used in Flow cytometry/Cell sorting to examine the role of BAFFR and TACI in T cell-independent antibody responses to an active Borrelia hermsii infection."AuthorsDickinson GS,Sun G,Bram RJ,Alugupalli KR3. The Journal of biological chemistryGene targeting study reveals unexpected expression of brain-expressed X-linked 2 in endocrine and tissue stem/progenitor cells in mice."17-4301 was used in Flow cytometry/Cell sorting to investigate the precise role and expression pattern of brain-expressed X-linked genes in endocrine and tissue stem/progenitor cells in mice."AuthorsIto K,Yamazaki S,Yamamoto R,Tajima Y,Yanagida A,Kobayashi T,Kato-Itoh M,Kakuta S,Iwakura Y,Nakauchi H,Kamiya A4. PloS oneGlatiramer acetate increases phagocytic activity of human monocytes in vitro and in multiple sclerosis patients."17-4301 was used in Flow cytometry/Cell sorting to demonstrate and evaluate a new mechanism of action for Glatiramer acetate treatment."AuthorsPul R,Morbiducci F,Škuljec J,Skripuletz T,Singh V,Diederichs U,Garde N,Voss EV,Trebst C,Stangel M

技术参数

产品应用 Flow;Ctrl

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