IFN gamma Monoclonal Antibody (XMG1.2), APC

货号：17-7311-82,17-7311-81

规格：100μg,50μg

价格：2432,1416

产品类型：流式抗体

品牌：Thermo Fisher

物种：人

宿主：大鼠

抗体亚型：IgG

克隆号：XMG1.2

荧光染料：APC

抗体类型:	单抗	同型对照：	Rat IgG1 kappa Isotype Control, APC, eBioscience™
免疫原性：		用法：	0.125µg/test(Flow）

Description: The XMG1.2 antibody reacts with mouse interferon-gamma. The XMG1.2 antibody is a neutralizing antibody. Mouse IFN-gamma is a 20 kDa factor produced by activated T, B and NK cells, and is an anti-viral and anti-parasitic cytokine. IFN-gamma, in synergy with other cytokines such as TNF-alpha, inhibits proliferation of normal and transformed cells. Immunomodulatory effects of IFN-gamma are exerted on a wide range of cell types expressing the high affinity receptors for IFN-gamma. Glycosylation of IFN-gamma does not affect its biological activity.Applications Reported: This XMG1.2 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: The APC conjugated XMG1.2 antibody has been tested by intracellular staining and flow cytometric analysis of stimulated mouse splenocytes and can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationIFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a dimeric protein consisting of two 146 amino acid subunits. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific. Human IFN gamma does not show cross-reactivity with mouse. IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines. Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway. IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma. IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. IFN gamma gene mutations are associated with aplastic anemia.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

IFN gamma Antibody (17-7311-82) Intracellular staining of stimulated mouse splenocytes. As expected based on known expression patterns, IFN gamma clone XMG1.2 stains a minor subset of CD4+ T cells and a larger subset of NK1.1+ NK cells with no staining observed without stimulation. Details: Mouse splenocytes were cultured in the presence of Protein Transport Inhibitors (500X) (Unstimulated, bottom row) or Cell Stimulation Cocktail (plus protein transport inhibitors, 500X) for 5 hours (Stimulated, top row). Cells were fixed and permeabilized with the IC Fixation and Permeabilization Buffer Set and protocol followed by intracellular staining with CD4 (clone RM4-5), NK1.1 (clone PK136) and IFN gamma (clone XMG1.2). Cells in the CD4+ (blue histogram) or NK1.1+ (purple histogram) gates were used for analysis. Cell Treatment validation info.Antibody target was verified by Cell Treatment to ensure the antibody binds to the antigen stated.

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参考文献：

1. International journal of medical sciencesT Cells Differentiation through T-bet and Eomesodermin in Response to Invasive Pulmonary Aspergillosis.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer2. F1000ResearchMyelin-specific T helper 17 cells promote adult hippocampal neurogenesis through indirect mechanisms.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer3. Nature communicationsRegulation of chitinase-3-like-1 in T cell elicits Th1 and cytotoxic responses to inhibit lung metastasis."17-7311 was used in Flow cytometry/Cell sorting to demonstrate that Chitinase-3-like-1 is a regulator of Th1 and CTL, which could be a potential anti-tumor immunity target."AuthorsKim DH,Park HJ,Lim S,Koo JH,Lee HG,Choi JO,Oh JH,Ha SJ,Kang MJ,Lee CM,Lee CG,Elias JA,Choi JM4. EBioMedicineA Multistage Subunit Vaccine Effectively Protects Mice Against Primary Progressive Tuberculosis, Latency and Reactivation.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer5. EBioMedicineDendritic Cells Aggravates Atherosclerosis via Th1 Cell Induction and Enhanced CCL5 Expression in Plaque Macrophages.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer

技术参数

产品应用 Flow

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