Ly-6G Monoclonal Antibody (1A8-Ly6g), APC

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Ly-6G Monoclonal Antibody (1A8-Ly6g), APC

货号:17-9668-82,17-9668-80

规格:100μg,25μg

价格:2996,934

产品类型:流式抗体

品牌:Thermo Fisher

物种:小鼠

宿主:大鼠

抗体亚型:IgG

克隆号:1A8-Ly6g

荧光染料:APC

抗体类型:

单抗

同型对照:

Rat IgG2a kappa Isotype Control, APC, eBioscience™

免疫原性:

用法:

0.5µg/test(Flow)

Description: The monoclonal antibody 1A8-Ly6g reacts with mouse Ly-6G, which is also known as Gr-1. Ly-6G is a 25-kDa GPI protein expressed exclusively by neutrophils. Studies suggest an important role in neutrophil infiltration, recruitment and migration. Ly-6G associates with B2 integrins CD11a and CD11b and can attenuate B2 integrin expression and function. The ligand for Ly-6G is currently unknown.Unlike the RB6-8C5 antibody, 1A8-Ly6g is reported to only bind to Ly-6G found on neutrophils. Moreover, 1A8-Ly6g has been used to deplete neutrophils and reported to have antagonist activity.Applications Reported: This 1A8-Ly6g antibody has been reported for use in flow cytometric analysis.Applications Tested: This 1A8-Ly6g antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationLy6G (Lymphocyte antigen 6 complex locus G6D) is a component of the myeloid differentiation antigen Gr-1, together with Ly6C. The Ly6G protein has a molecular weight of approximately 21-25 kDa and is expressed by myeloid derived cells in the bone marrow. Ly6G is a good marker for detection of peripheral neutrophils, monocytes and granulocytes. Expression of Gr-1 in bone marrow correlates with granulocyte differentiation and maturation. The physiological role of Ly6G remains unclear but studies have shown its involvement in anti-tumor response, and studies with neutrophils suggest that Ly6G ligation may have an inhibitory effect on local immune responses.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

Ly-6G Antibody (17-9668-82) in Flow Staining of C57Bl/6 bone marrow cells with Anti-Human/Mouse CD45R (B220) FITC (Product # 11-0452-82) and 0.25 µg of Rat IgG2a K Isotype Control APC (Product # 17-4321-81) (left) or 0.25 µg of Anti-Mouse Ly-6G (Gr-1) APC (right)

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参考文献:

1. Frontiers in immunologyATP-Induced Inflammasome Activation and Pyroptosis Is Regulated by AMP-Activated Protein Kinase in Macrophages.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer2. PloS onep110γ/δ Double-Deficiency Induces Eosinophilia and IgE Production but Protects from OVA-Induced Airway Inflammation."17-9668 was used in Flow cytometry/Cell sorting to conclude that increased levels of eosinophils and IgE in p110γ/δ-/- mice do not abolish the protective effect of p110γ/δ-deficiency against OVA-induced allergic airway inflammation."AuthorsMothes B,Bucher K,Ammon-Treiber S,Schwab M,Piekorz RP,Hirsch E,Nürnberg B,Beer-Hammer S3. GastroenterologySphingosine-1-Phosphate Prevents Egress of Hematopoietic Stem Cells From Liver to Reduce Fibrosis."17-9668 was used in Flow cytometry/Cell sorting to investigate the role of sphingosine-1-phosphate in the response of hematopoietic stem cells to liver fibrosis in mice."AuthorsKing A,Houlihan DD,Kavanagh D,Haldar D,Luu N,Owen A,Suresh S,Than NN,Reynolds G,Penny J,Sumption H,Ramachandran P,Henderson NC,Kalia N,Frampton J,Adams DH,Newsome PN4. Journal of diabetes researchPostnatal Hematopoiesis and Gut Microbiota in NOD Mice Deviate from C57BL/6 Mice."17-9668 was used in Flow cytometry/Cell sorting to investigate whether NOD mice differ to C57BL/6 mice with respect to postnatal microbiota and/or hematopoiesis."AuthorsDamlund DS,Metzdorff SB,Hasselby JP,Wiese M,Lundsager M,Nielsen DS,Buschard KS,Hansen AK,Frøkiær H5. Infection and immunityReal-Time Imaging of Interactions of Neutrophils with Cryptococcus neoformans Demonstrates a Crucial Role of Complement C5a-C5aR Signaling."17-9668 was used in Flow cytometry/Cell sorting to characterise the dynamic interactions of neutrophils with C. neoformans, showing a crucial role of complement C5a-C5aR signalling."AuthorsSun D,Zhang M,Liu G,Wu H,Zhu X,Zhou H,Shi M
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