Mouse anti Bovine CD21 monoclonal antibody, clone CC51
货号：MCA5953F
规格：0.1 mg
价格：3766
产品类型：流式抗体
品牌：Bio-Rad
抗原：CD21
物种：Bovine
宿主：小鼠
抗体亚型：IgG2b
克隆号：CC51
荧光染料：FITC
| 产品介绍 | | |
| Mouse anti Bovine CD21 monoclonal antibody, clone CC51recognizes the bovine homologue of the human CD21 cell surface antigen, a 145 kDa single pass type I membrane glycoprotein containing multiplesushidomains. CD21 is also known as complement receptor type 2 (CR2). In cattle CD21 expression is restricted to B-cells (Naessenset al.1990). CD21 may be expressed on B-cells as either a long or a short form (Pringleet al.2012).Mouse anti Bovine CD21, clone CC51 demonstrates cross reactivity with porcine and provides a reliable marker for porcine B-Cells (Sinkoraet. al.2013). In addition to clone CC51, clone CC21 (MCA1424GA) which has been demonstrated to recognise CD21 in a range of ruminant and other species is also available from Bio-Rad. |
| 产品详情 | | |
| Target Species | Bovine |
| Product Form | Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized |
| Reconstitution | Reconstitute with 1.0 ml distilled waterCare should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. |
| Preparation | Purified IgG prepared by affinity chromatography on Protein A |
| Buffer Solution | Phosphate buffered saline |
| Immunogen | Bovine (Friesian cattle) mesenteric lymph node cells |
| Preservative Stabilisers | 0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin5% Sucrose |
| Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
| RPE 488nm laser | 496 | 578 |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| Store at +4℃. DO NOT FREEZE.This product should be stored undiluted. This product is photosensitive and should be protected from light. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Flow Cytometry | √ | | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 文献 |
| 1.Sinkora, M.et al.(2014) The comparative profile of lymphoid cells and the T and B cell spectratype of germ-free piglets infected with viruses SIV, PRRSV or PCV2.Vet Res. 45: 91.2.Sinkora, Met al.(2013) Different anti-CD21 antibodies can be used to discriminate developmentally and functionally different subsets of B lymphocytes in circulation of pigs.Dev Comp Immunol. 39: 409-18.3.Tenaya I.W.et al.(2012) Flow cytometric analysis of lymphocyte subset kinetics in Bali cattle experimentally infected with Jembrana disease virus.Vet Immunol Immunopathol. 149: 167-76.4.Denham S.et al.(1994) Monoclonal antibodies recognising differentiation antigens on porcine B cells.Vet Immunol Immunopathol. 43: 259-67. |
技术参数 产品应用 Flow
产品类型 Monoclonal Antibody
推荐用于 Flow
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