Rat IgG2b kappa Isotype Control

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Rat IgG2b kappa Isotype Control

货号:14-4031-82,14-4031-81,14-4031-85

规格:100μg,50μg,500μg

价格:971,605,1450

产品类型:大鼠同型对照

品牌:eBioscience

物种:其它

宿主:大鼠

抗体亚型:IgG2b, kappa

克隆号:eB149/10H5

荧光染料:其它

抗体类型:

单抗

同型对照:

IgG2b, kappa

免疫原性:

用法:

Assay-Dependent (Ctrl);Assay-Dependent (Flow);Assay-Dependent (IHC)

Description: The rat IgG2b, kappa monoclonal antibody is useful as an isotype control immunoglobulin.Applications Reported: The monoclonal rat IgG2b kappa isotype control has been reported for use in flow cytometric analysis, immunohistochemistry, and immunocytochemistry.Applications Tested: Purified Rat IgG2b Isotype Control has been tested by flow cytometric analysis of mouse splenocyte suspensions. It should be used at the same concentration as the experimental antibody.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationThe isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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参考文献:

1. Journal of neuroinflammationNOX2 deficiency alters macrophage phenotype through an IL-10/STAT3 dependent mechanism: implications for traumatic brain injury."14-4031 was used as a Control in experiments to investigate the signalling pathways that regulate NOX2-dependent macrophage/microglial phenotype switching in the TBI brain."AuthorsBarrett JP,Henry RJ,Villapol S,Stoica BA,Kumar A,Burns MP,Faden AI,Loane DJ2. PloS oneFetuin-A-containing calciprotein particles reduce mineral stress in the macrophage."14-4031 was used as a Control in experiments to demonstrate that calciprotein particles induce expression and secretion of tumour necrosis factor-α, interleukin-1β in murine RAW 264.7 macrophages."AuthorsSmith ER,Hanssen E,McMahon LP,Holt SG3. Journal of immunology (Baltimore, Md. : 1950)Involvement of an NKG2D ligand H60c in epidermal dendritic T cell-mediated wound repair."14-4031 was used as a Control in experiments to demonstrate a general role for H60c in the maintenance of epithelial integrity in a wide range of tissues."AuthorsYoshida S,Mohamed RH,Kajikawa M,Koizumi J,Tanaka M,Fugo K,Otsuka N,Maenaka K,Yagita H,Chiba H,Kasahara M4. Molecular cancerMetalloprotease-disintegrin ADAM12 actively promotes the stem cell-like phenotype in claudin-low breast cancer."14-4031 was used in Flow cytometry/Cell sorting to investigate whether ADAM12 plays an active role in promoting the cancer stem cell phenotype in breast cancer cells."AuthorsDuhachek-Muggy S,Qi Y,Wise R,Alyahya L,Li H,Hodge J,Zolkiewska A4. OncoimmunologyPoly(I:C) and CpG-ODN combined aerosolization to treat lung metastases and counter the immunosuppressive microenvironment."14-4031 was used in Flow cytometry/Cell sorting to evaluated aerosol delivery CpG-ODN combined with Poly(I:C) in the treatment of B16 melanoma lung metastases in C57BL/6 mice."AuthorsLe Noci V,Tortoreto M,Gulino A,Storti C,Bianchi F,Zaffaroni N,Tripodo C,Tagliabue E,Balsari A,Sfondrini L5. Nature communicationsRoles of lymphatic endothelial cells expressing peripheral tissue antigens in CD4 T-cell tolerance induction."14-4031 was used in Flow cytometry/Cell sorting to demonstrate that when β-galactosidase (β-gal) is expressed in LECs, β-gal-specific CD8 T cells undergo deletion via the PD-1/PD-L1 and LAG-3/MHC-II pathways."AuthorsRouhani SJ,Eccles JD,Riccardi P,Peske JD,Tewalt EF,Cohen JN,Liblau R,Mäkinen T,Engelhard VH

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