OCT4 Polyclonal Antibody
货号：PA1-16943
规格：100 µL
价格：5548
产品类型：流式抗体
品牌：Thermo Fisher
抗原：OCT4
物种：人/小鼠/大鼠
宿主：兔
抗体亚型：IgG
荧光染料：Purified
抗体类型: | 多抗 | 同型对照： | IgG |
免疫原性： | Synthetic peptide between amino acids 100-200 of the human OCT4 protein. | 用法： | 1:50-1:200(Flow)；1:50-1:200(ICC);1:50-1:200(IF);1:1000(WB) |
Product Specific InformationThis antibody is expected to react with swine, feline and bovine samples based on a 93% sequence homology.Target InformationStem cells are crucial ingredients in the progressive restriction of cellular developmental capacity during mammalian embryogenesis. Until recently, only embryonic stem cells (ESC) had been shown to exhibit the potential to differentiate into cells of the all three germ layers. However, adult stem cells (ASC) can regain pluripotency (potential to differentiate into various lineages) if transferred from one stem-cell microenvironment into another. The transcription factor OCT4, expressed in ESCs and germ cells, is strongly implicated in the process of maintaining as well as regaining stem-cell pluripotency and functions as a key regulator of mammalian germline development.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
数据 |
| OCT4 Antibody (PA1-16943) in FlowFACS staining of NTERA-2 cells using Product # PA1-16943 at a 1:50 dilution detected using Dylight-488 conjugated goat anti-rabbit secondary antibody.OCT4 Antibody (PA1-16943) in FlowFACS staining of NTERA-2 cells using Product # PA1-16943 at a 1:50 dilution detected using Dylight-488 conjugated goat anti-rabbit secondary antibody.OCT4 Antibody (PA1-16943) in WBWestern blot analysis was performed on modified whole cell extracts (1% SDS) (30 µg lysate) of (Fig. a ) NCCIT (Lane 1), NTERA-2 (Lane 2) and HeLa (Lane 3), (Fig. b) Human iPSCs (Lane 1) and Human iPSCs derived definitive endoderm (Lane 2). The blot was probed with Anti- OCT4 Polyclonal Antibody (Product # PA1-16943, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 38 kDa band corresponding to OCT4 was observed across the cell lines tested except for HeLa (Fig. a) and human iPSC derived definitive endoderm (Fig. b) which is reported to be negative for OCT4 expression. |
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参考文献： |
| 1.Reproduction in domestic animals = Zuchthygiene The endometrium of cycling cows contains populations of putative mesenchymal progenitor cells. "PA1-16943 was used in western blot to study the potential existence of mesenchymal progenitor cells in cow endometrium" AuthorsCabezas J,Lara E,Pacha P,Rojas D,Veraguas D,Saravia F,Rodríguez-Alvarez L,Castro FO |
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