MHC Class I (H-2Kd) Monoclonal Antibody (SF1-1.1.1), APC

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MHC Class I (H-2Kd) Monoclonal Antibody (SF1-1.1.1), APC

货号:17-5957-82,17-5957-80

规格:100μg,25μg

价格:2431,934

产品类型:流式抗体

品牌:Thermo Fisher

物种:小鼠

宿主:小鼠

抗体亚型:IgG

克隆号:SF1-1.1.1

荧光染料:APC

抗体类型:

单抗

同型对照:

IgG2a, kappa

免疫原性:

用法:

0.125 µg/test(Flow)

Description: This SF1-1.1.1 monoclonal antibody reacts with the H-2Kd MHC class I alloantigen. H-2Kd is involved in antigen presentation to T cells expressing CD3/TCR and CD8. Reactivity to other haplotypes (e.g. b, j, k, p, q, s, and v) has not been observed.Applications Reported: This SF1-1.1.1 antibody has been reported for use in flow cytometric analysis.Applications Tested: This SF1-1.1.1 antibody has been tested by flow cytometric analysis on Balb/c splenocytes. This antibody can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationThis protein is an MHC class I alloantigen, which is involved in antigen presentation to T cells expressing CD3/TCR and CD8.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

MHC Class I (H-2Kd) Antibody (17-5957-82) in FlowStaining of C57BL/6 (left) or BALB/c (right) splenocytes with 0.06 µg of Mouse IgG2a K Isotype Control APC (Product # 17-4724-81) (blue histogram) or 0.06 µg of Anti-Mouse MHC Class I (H-2Kd) APC (purple histogram). Total viable cells were used for analysis.

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参考文献:

1. Journal of translational medicineA therapeutic cancer vaccine against GL261 murine glioma.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer2. Cancer researchDifferential WNT activity in colorectal cancer confers limited tumorigenic potential and is regulated by MAPK signaling."17-5957 was used in Flow cytometry/Cell sorting to suggest that on its own, high WNT activity may not be a reliable signifier of tumor-initiating potential or stem-like potential."AuthorsHorst D,Chen J,Morikawa T,Ogino S,Kirchner T,Shivdasani RA3. Stem cells (Dayton, Ohio)ALDH1A isozymes are markers of human melanoma stem cells and potential therapeutic targets."17-5957 was used in Flow cytometry/Cell sorting to demonstrate the existence of melanoma cells that fulfil the criteria for CSCs."AuthorsLuo Y,Dallaglio K,Chen Y,Robinson WA,Robinson SE,McCarter MD,Wang J,Gonzalez R,Thompson DC,Norris DA,Roop DR,Vasiliou V,Fujita M
技术参数

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