NK1.1 Monoclonal Antibody (PK136), APC

货号：17-5941-82,17-5941-63,17-5941-81

规格：100μg,10μg,50μg

价格：2934,408,1822

产品类型：流式抗体

品牌：Thermo Fisher

物种：小鼠

宿主：小鼠

抗体亚型：IgG

克隆号：PK136

荧光染料：APC

抗体类型:	单抗	同型对照：	Mouse IgG2a kappa Isotype Control, APC, eBioscience™
免疫原性：		用法：	0.125µg/test(Flow）

Description: The PK136 monoclonal antibody reacts with mouse NK1.1, an antigen expressed by natural killer cells and a subset of T cells in the NK1.1 mouse strains including C57BL and NZB. Several commonly used laboratory mouse strains such as BALB/c, SJL, AKR, CBA, C3H and A do not express the NK1.1 antigen. For detection of NK cells in these strains the monoclonal antibody DX5 (Cat. No. 14-5971) should be used. Simultaneous staining of C57BL/6 spleen cells with PK136 and DX5 reveals coexpression of both markers by a majority of cells as well as presence of small populations of DX5+PK136- and DX5-PK136+ cells.Applications Reported: PK136 has been reported for use in flow cytometric analysis.Applications Tested: The PK136 antibody has been tested by flow cytometric analysis of C57Bl/6 mouse splenocytes and can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationNK1.1 (Killer cell lectin-like receptor subfamily B, member 1, KLRB1, NKR-P1A, CD161, cluster of differentiation 161), refers to Natural Killer (NK) cells, lymphocytes that mediate cytotoxicity and secrete cytokines after immune stimulation. Several genes of the C-type lectin superfamily, including the rodent NKRP1 family of glycoproteins, are expressed by NK cells and may be involved in the regulation of NK cell function. The KLRB1 protein contains an extracellular domain with several motifs characteristic of C-type lectins, a transmembrane domain, and a cytoplasmic domain. The KLRB1 protein, NKR-P1A or CD161, is classified as a type II membrane protein because it has an external C terminus. NKR-P1A, the receptor encoded by the KLRB1 gene, recognizes Lectin Like Transcript-1 (LLT1) as a functional ligand.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

NK1.1 Antibody (17-5941-82) in Flow Staining of C57BL/6 splenocytes with staining buffer (autofluorescence) (open histogram) or 0.06 µg of Anti-Mouse NK1.1 APC (filled histogram). Total viable cells were used for analysis

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参考文献：

1. LeukemiaA TLR7 agonist enhances the antitumor efficacy of obinutuzumab in murine lymphoma models via NK cells and CD4 T cells.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer2. Frontiers in immunologyMicroRNA-7 Deficiency Ameliorates the Pathologies of Acute Lung Injury through Elevating KLF4."17-5941 was used in Flow cytometry/Cell sorting to study the role of microRNA-7 in acute lung injury, allowing the development of new therapeutic strategies against inflammatory lung disease."AuthorsZhao J,Chen C,Guo M,Tao Y,Cui P,Zhou Y,Qin N,Zheng J,Zhang J,Xu L3. PloS oneFavorable alteration of tumor microenvironment by immunomodulatory cytokines for efficient T-cell therapy in solid tumors. By clicking the link above, you will be redirected to the Benchsci website.Disclaimer4. Journal of the American Society of Nephrology : JASNHypoxia-Inducible Factor-2α Limits Natural Killer T Cell Cytotoxicity in Renal Ischemia/Reperfusion Injury.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer5. Nature communicationsNK cell development requires Tsc1-dependent negative regulation of IL-15-triggered mTORC1 activation.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer

技术参数

产品应用 Flow

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