Mouse IgG2a kappa Isotype Control, APC
货号：17-4724-81
规格：50μg
价格：1168
产品类型：小鼠同型对照
品牌：eBioscience
物种：其它
宿主：小鼠
抗体亚型：IgG2a, kappa
克隆号：eBM2a
荧光染料：APC
抗体类型: | 同型对照 | 同型对照： | |
浓度： | 0.2 mg/mL | 用法： | Assay-Dependent (Ctrl)； 1 µg/test(Flow） |
Description: This is a monoclonal mouse IgG2a, kappa antibody. It is used as an isotype control for mouse IgG2a antibodies.Applications Reported: This mouse IgG2a isotype control has been reported for use in flow cytometric analysis.Applications Tested: The APC conjugated mouse IgG2a isotype control has been tested by flow cytometric analysis of normal human peripheral blood cells and can be used at less than or equal to 1 µg/million cells (100 µL blood). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationThe isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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参考文献： |
| 1. PloS oneEstradiol alters the immune-responsiveness of cervical epithelial cells stimulated with ligands of Toll-like receptors 2 and 4."17-4724 was used in Flow cytometry/Cell sorting to demonstrate that Estradiol alters the immune-responsiveness of HECECs to TLR2 and TLR4 ligands by TLR subtype and duration of exposure."AuthorsS Lashkari B,Anumba DO2. Molecular visionA comprehensive flow-cytometric analysis of graft infiltrating lymphocytes, draining lymph nodes and serum during the rejection phase in a fully allogeneic rat cornea transplant model."17-4724 was used in Flow cytometry/Cell sorting to define the immunologic reaction toward corneal allografts."AuthorsMaenz M,Morcos M,Ritter T3. The Journal of infectious diseasesB and T lymphocyte attenuator down-regulation by HIV-1 depends on type I interferon and contributes to T-cell hyperactivation."17-4724 was used as a Control in experiments to characterise B and T lymphocyte attenuator expression and function during HIV-1 infection."AuthorsZhang Z,Xu X,Lu J,Zhang S,Gu L,Fu J,Jin L,Li H,Zhao M,Zhang J,Wu H,Su L,Fu YX,Wang FS |
QQ:1749072012