Ly-6G/Ly-6C Monoclonal Antibody (RB6-8C5), APC

货号：17-5931-81,17-5931-82

规格：50μg,100μg

价格：1822,3035

产品类型：流式抗体

品牌：Thermo Fisher

物种：小鼠

宿主：大鼠

抗体亚型：IgG2b, kappa

克隆号：RB6-8C5

荧光染料：APC

抗体类型:	单抗	同型对照：	Rat IgG2b kappa Isotype Control, APC, eBioscience™
免疫原性：		用法：	0.125µg/test(Flow）

Description: The RB6-8C5 monoclonal antibody reacts with mouse Ly-6G, a 21-25 kDa protein also known as the myeloid differentiation antigen Gr-1. A GPI-linked protein, Gr-1 is expressed by the myeloid lineage in a developmentally regulated manner in the bone marrow. While monocytes only express Gr-1 transiently during their bone marrow development, the expression of Gr-1 on bone marrow granulocytes as well as on peripheral neutrophils is a good marker for these populations.eBioscience testing indicates that in the bone marrow and lysed whole blood, the antibody clone RB6-8C5 also stains cells that express the highest levels of Ly6c (as defined by staining with antibody clone HK1.4). It is recommended that 1A8-Ly6G (cat. 9668) be used when looking at Ly-6G specific targets.Applications Reported: The RB6-8C5 antibody has been reported for use in flow cytometric analysis.Applications Tested: The RB6-8C5 antibody has been tested by flow cytometric analysis of mouse bone marrow cells and splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationGranulocytes are a category of white blood cells characterised by the presence of granules in their cytoplasm. They are also called polymorphonuclear leukocytes (PMN or PML) because of the varying shapes of the nucleus, which is usually lobed into three segments. In common parlance, the term polymorphonuclear leukocyte often refers specifically to neutrophil granulocytes, the most abundant of the granulocytes. Granulocytes or PMN are released from the bone marrow by the regulatory complement proteins.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

Ly-6G/Ly-6C Antibody (17-5931-82) in Flow Staining of C57BL/6 bone marrow cells with 0.125 µg of Rat IgG2b kappa Isotype Control APC (Product # 17-4031-82) (blue histogram) or 0.125 µg of Anti-Mouse Ly-6G (Gr-1) APC (purple histogram). Total viable cells were used for analysis.

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参考文献：

1. RNA biologyADAR1 deletion induces NFκB and interferon signaling dependent liver inflammation and fibrosis. By clicking the link above, you will be redirected to the Benchsci website.Disclaimer2. PLoS geneticsMiR-125a Is a critical modulator for neutrophil development.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer3. International journal of molecular medicineSilybin attenuates LPS-induced lung injury in mice by inhibiting NF-κB signaling and NLRP3 activation.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer4. PloS oneEnterococcus durans TN-3 Induces Regulatory T Cells and Suppresses the Development of Dextran Sulfate Sodium (DSS)-Induced Experimental Colitis."17-5931 was used in Flow cytometry/Cell sorting to investigate the effects of E. durans TN-3 on the development of dextran sulfate sodium colitis."AuthorsKanda T,Nishida A,Ohno M,Imaeda H,Shimada T,Inatomi O,Bamba S,Sugimoto M,Andoh A5. Nature medicineThe creatine kinase pathway is a metabolic vulnerability in EVI1-positive acute myeloid leukemia."17-5931 was used in Flow cytometry/Cell sorting to investigate the effect of MECOM on cellular metabolism."AuthorsFenouille N,Bassil CF,Ben-Sahra I,Benajiba L,Alexe G,Ramos A,Pikman Y,Conway AS,Burgess MR,Li Q,Luciano F,Auberger P,Galinsky I,DeAngelo DJ,Stone RM,Zhang Y,Perkins AS,Shannon K,Hemann MT,Puissant A,Stegmaier K

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