MHC Class II (I-A/I-E) Monoclonal Antibody (M5/114.15.2), APC, eBioscience™

货号：17-5321-81,17-5321-82

规格：50μg,100μg

价格：1674,2732

产品类型：流式抗体

品牌：Thermo Fisher

物种：小鼠

宿主：大鼠

抗体亚型：IgG2b, kappa

克隆号：M5/114.15.2

荧光染料：APC

抗体类型:	单抗	同型对照：	Rat IgG2b kappa Isotype Control, APC, eBioscience™
免疫原性：		用法：	0.03 µg/test(Flow）

Description: The M5/114.15.2 monoclonal antibody reacts with the mouse major histocompatibility complex class II, both I-A and I-E subregion-encoded glycoproteins (I-A b, I-A d, I-A q, I-E d, I-E k, not I-A f, I-A k, or I-A s). It detects a polymorphic determinant present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying the H-2 b, H-2 d, H-2 q, H-2 p, H-2 r and H-2 u but not from mice carrying the H-2 s or H-2 f haplotypes. The M5/114 mAb is reported to inhibit I-A-restricted T cell responses of the H-2 b, H-2 d, H-2 q, H-2 u but not H-2 f, H-2 k, or H-2 s haplotypes.Applications Reported: M5/114.15.2 has been reported for use in flow cytometric analysis.Applications Tested: The M5/114.15.2 antibody has been tested by flow cytometric analysis of mouse splenocytes and can be used at less than or equal to 0.03 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationMHC (major histocompatibility complex) class II molecules are transmembrane glycoproteins expressed on the surface of professional antigen-presenting cells, such as macrophages, dendritic cells and B cells. Before their exposition on the cell surface, the MHC class II molecules react with endocytosed exogenous antigens, which are then presented to the T cells. The antigen-binding grove between MHC class II alpha and beta chain is open at both ends and is 15-24 amino acid residues long.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

MHC Class II (I-A/I-E) Antibody (17-5321-81) in Flow Staining of C57BL/6 splenocytes with Anti-Mouse CD3e FITC (Product # 11-0031-82) and 0.015 µg of Rat IgG2b K Isotype Control APC (Product # 17-4031-82) (left) or 0.015 µg of Anti-Mouse MHC Class II (I-A/I-E) APC (right).Cells in the lymphocyte gate were used for analysis.

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参考文献：

1. PloS oneRole of a novel immune modulating DDR2-expressing population in silica-induced pulmonary fibrosis.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer2. The Journal of clinical investigationA CCR4 antagonist reverses the tumor-promoting microenvironment of renal cancer."17-5321 was used in Flow cytometry/Cell sorting to determine the effect of CCR4 antagonism on human renal cell carcinoma."AuthorsBerlato C,Khan MN,Schioppa T,Thompson R,Maniati E,Montfort A,Jangani M,Canosa M,Kulbe H,Hagemann UB,Duncan AR,Fletcher L,Wilkinson RW,Powles T,Quezada SA,Balkwill FR3. eLifeInternalization and presentation of myelin antigens by the brain endothelium guides antigen-specific T cell migration.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer4. Nature neuroscienceMicroglial brain region-dependent diversity and selective regional sensitivities to aging.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer5. AgingStroke sensitivity in the aged: sex chromosome complement vs. gonadal hormones.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer

技术参数

产品应用 Flow

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