Armenian Hamster IgG Isotype Control, APC

货号：17-4888-81,17-4888-82

规格：50μg,100μg

价格：1237,2055

产品类型：同型对照

品牌：Thermo Fisher

物种：其它

宿主：仓鼠

抗体亚型：IgG

克隆号：eBio299Arm

荧光染料：eFluor 506

抗体类型:	仓鼠同型对照	同型对照：	IgG
浓度：	0.2 mg/mL	用法：	Assay-Dependent (Ctrl)； Assay-Dependent (Flow)

Description: The eBio299Arm monoclonal antibody is useful as an isotype control immunoglobulin.Applications Reported: This eBio299Arm antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBio299Arm antibody has been tested by flow cytometric analysis. Isotype control should be used at the same concentration as experimental antibody.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationThe isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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参考文献：

1. Cell adhesion and migrationExpression of kindlin-3 in melanoma cells impedes cell migration and metastasis."17-4888 was used in Flow cytometry/Cell sorting to demonstrate that aberrant expression of kindlin-3 could lead to imbalanced RhoGTPases signaling that impedes rather than promotes cell migration."AuthorsFeng C,Wee WK,Chen H,Ong LT,Qu J,Tan HF,Tan SM2. Cell transplantationControl of the Survival and Growth of Human Glioblastoma Grafted Into the Spinal Cord of Mice by Taking Advantage of Immunorejection."17-4888 was used in Flow cytometry/Cell sorting to establish a glioblastoma multiforme xenograft model mouse."AuthorsItakura G,Kobayashi Y,Nishimura S,Iwai H,Takano M,Iwanami A,Toyama Y,Okano H,Nakamura M3. Stem cell researchMigration of CD11b+ accessory cells during murine lung regeneration."17-4888 was used in Flow cytometry/Cell sorting to characterise the factors contributing to neoalveolarization within 21 days of pneumonectomy."AuthorsChamoto K,Gibney BC,Lee GS,Ackermann M,Konerding MA,Tsuda A,Mentzer SJ

技术参数

产品应用 Flow

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