CD317 (BST2, PDCA-1) Monoclonal Antibody (eBio927), APC

货号：17-3172-80,17-3172-82

规格：25μg,100μg

价格：1029,3090

产品类型：流式抗体

品牌：Thermo Fisher

物种：小鼠

宿主：大鼠

抗体亚型：IgG2b, kappa

克隆号：eBio927

荧光染料：APC

抗体类型:	单抗	同型对照：	Rat IgG2b kappa Isotype Control, APC, eBioscience™
免疫原性：		用法：	0.25 µg/test(Flow）

Description: The eBio927 monoclonal antibody reacts with PDCA-1 (BST2, CD317), a specific marker of plasmacytoid dendritic cells (pDC), also known as type I IFN-producing cells (IPC) in the naive mouse. Mouse IPCs are typically CD11c+, CD11b-, B220+, Ly-6C+, and CD62L+. PDCA-1 is predominantly expressed by IPCs in the naive mouse which represents a very minor population (<0.5%) of splenocytes. It is upregulated on numerous cell types following stimulation which triggers an IFN response. PDCA-1 cycles between cell surface and intracellular compartments and may function to regulate trafficking of secreted cytokines. PDCA-1 (BST2) is the protein recognized by antibody 120G8. The eBio927 monoclonal antibody has also been shown to have functional activity. The epitope recognized by eBio927 is distinct from eBio129c; thus, the antibodies can be used to costain, purify and identify pDCs.Applications Reported: This eBio927 antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBio927 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationBST2, also known as CD317, HM1.24 antigen, DAMP-2 and recently designated tetherin, is an integral membrane protein with a novel topology. The specific function of the BST2, encoded by the bone marrow stromal cell antigen 2 gene, is undetermined; however, it may play a role in pre-B-cell growth, rheumatoid arthritis, and in antiretroviral defense, that blocks release of retrovirus from the cell surface. BST2 is highly expressed during B-cell development, from pro-B precursors to plasma cells, T-cells, monocytes, NK cells and DCs (at protein level).For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

CD317 (BST2, PDCA-1) Antibody (17-3172-82) in Flow Staining of SJL splenocytes with Anti-Human/Mouse CD45R (B220) FITC (Product # 11-0452-82) and 0.125 µg of Rat IgG2b K Isotype Control APC (Product # 17-4031-82) (left) or 0.125 µg of Anti-Mouse CD317 (BST2, PDCA-1) APC (right). Total viable cells were used for analysis.

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参考文献：

1.Nature immunologyBatf3 maintains autoactivation of Irf8 for commitment of a CD8α(+) conventional DC clonogenic progenitor."17-3172 was used in Flow cytometry/Cell sorting to investigate the role of the transcription factors Batf3 and IRF8 in the development of CD8α(+) conventional dendritic cells (cDCs).."AuthorsGrajales-Reyes GE,Iwata A,Albring J,Wu X,Tussiwand R,Kc W,Kretzer NM,Briseño CG,Durai V,Bagadia P,Haldar M,Schönheit J,Rosenbauer F,Murphy TL,Murphy KM2. PLoS pathogensEarly virus-host interactions dictate the course of a persistent infection."17-3172 was used in Flow cytometry/Cell sorting to investigate the very early host-virus interactions that determine the establishment of viral persistence."AuthorsSullivan BM,Teijaro JR,de la Torre JC,Oldstone MB3. Nature immunologyThe miR-126-VEGFR2 axis controls the innate response to pathogen-associated nucleic acids."17-3172 was used in Flow cytometry/Cell sorting to identify the miR-126-VEGFR2 axis as an important regulator of the innate response that operates through multiscale control of pDCs."AuthorsAgudo J,Ruzo A,Tung N,Salmon H,Leboeuf M,Hashimoto D,Becker C,Garrett-Sinha LA,Baccarini A,Merad M,Brown BD4. PloS oneBcl11a controls Flt3 expression in early hematopoietic progenitors and is required for pDC development in vivo."17-3172 was used in Flow cytometry/Cell sorting to investigate the role of Bcl11a in the development of dendritic cells, showing that it controls Flt3 expression in early hematopoietic progenitors and is required for pDC development."AuthorsWu X,Satpathy AT,Kc W,Liu P,Murphy TL,Murphy KM5. The Journal of experimental medicineZbtb46 expression distinguishes classical dendritic cells and their committed progenitors from other immune lineages."17-3172 was used in Flow cytometry/Cell sorting to identify Zbtb46 as a transcription factor selectively expressed by classical DCs and their committed progenitors."AuthorsSatpathy AT,KC W,Albring JC,Edelson BT,Kretzer NM,Bhattacharya D,Murphy TL,Murphy KM

技术参数

产品应用 Flow

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