CD223 (LAG-3) Monoclonal Antibody (3DS223H), APC
货号：17-2239-41,17-2239-42
规格：25 Tests,100 Tests
价格：1577,3894
产品类型：流式抗体
品牌：Thermo Fisher
物种：人/恒河猴
宿主：小鼠
抗体亚型：IgG1, kappa
克隆号：3DS223H
荧光染料：APC
抗体类型: | 单抗 | 同型对照： | Mouse IgG1 kappa Isotype Control, APC, eBioscience™ |
免疫原性： | | 用法： | 5uL（0.06µg）/test(Flow） |
Description: This 3DS223H monoclonal antibody recognizes human CD223 also known as Lymphocyte Activation Gene 3 (LAG-3). LAG-3 is a 70-kDa surface glycoprotein belonging to the Ig superfamily with homology to CD4. LAG-3 binds to MHC class II with higher affinity than CD4 and is thought to be involved in the negative regulation of T cell activation and homeostatic proliferation. Surface expression of LAG-3 has been reported on activated T cells (including regulatory T cells) and NK cells. CD8+ T cells usually express LAG-3 at significantly higher levels than CD4+ T cells. Coexpression of LAG-3 and CD49b has been proposed to identify human and mouse Type 1 regulatory T cells (Tr1 cells).This 3DS223H antibody will recognize a formaldehyde-fixed epitope.Applications Reported: This 3DS223H antibody has been reported for use in flow cytometric analysis.Applications Tested: This 3DS223H antibody has been pre-titrated and tested by flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 µL (0.06 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationLAG-3 is a 70-kDa surface glycoprotein belonging to the Ig superfamily with homology to CD4. LAG-3 binds to MHC class II with higher affinity than CD4 and is thought to be involved in the negative regulation of T cell activation and homeostatic proliferation. Surface expression of LAG-3 has been reported on activated T cells (including regulatory T cells) and NK cells. CD8+ T cells usually express LAG-3 at significantly higher levels than CD4+ T cells. Coexpression of LAG-3 and CD49b has been proposed to identify human and mouse Type 1 regulatory T cells (Tr1 cells).For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
数据 |
| CD223 (LAG-3) Antibody (17-2239-42) in Flow Normal human peripheral blood cells were stimulated for 3 days with Human IL-2 Recombinant Protein (Product # 14-8029-81), Anti-Human CD3, and Anti-Human CD28 Functional Grade Purifieds (Product # 16-0037-81 and Product # 16-0289-81). These cells were then surface stained with Anti-Human CD8a FITC (Product # 11-0087-42) and Mouse IgG1 K Isotype Control APC (Product # 17-4714-81) (left) or Anti-Human CD223 (LAG-3) APC (right). Viable cells, as determined by Fixable Viability Dye eFluor® 450 (Product # 65-0863-14), in the lymphocyte gate were used for analysis. |
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参考文献： |
| 1.Nature communications Follicular regulatory T cells impair follicular T helper cells in HIV and SIV infection. By clicking the link above, you will be redirected to the Benchsci website.Disclaimer |
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