CD69 Monoclonal Antibody (H1.2F3), APC, eBioscience

货号：17-0691-80,17-0691-82

规格：25μg,100μg

价格：967,2988

产品类型：流式抗体

品牌：eBioscience

抗原：CD69

物种：小鼠

宿主：仓鼠

抗体亚型：IgG

克隆号：H1.2F3

荧光染料：APC

抗体类型:	流式抗体	同型对照：	Armenian Hamster IgG Isotype Control (eBio299Arm), APC, eBioscience
浓度：	0.2 mg/mL	用法：	0.25 µg/test(Flow）

The H1.2F3 monoclonal antibody reacts with mouse CD69, also known as very early activation antigen (VEA). CD69 is approximately 35 kDa and is expressed on the surface as a disulfide-linked dimer. While a small subset of lymphocytes in the thymus, spleen and lymph nodes express this antigen, activation of both T and B cells rapidly upregulates the surface expression of CD69, suggesting a role for CD69 in lymphocyte development and activation.Applications Reported: This H1.2F3 antibody has been reported for use in flow cytometric analysis.Applications Tested: This H1.2F3 antibody has been tested by flow cytometric analysis of stimulated mouse splenic cell cultures. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD69 (AIM, Active Inducer Molecule) is a gp28/34 disulfide bonded homodimer with a molecular weight of 60 kDa under non-reducing conditions. CD69 contains one or two N linked oligosacaride and the molecule is present on activated platelets. In normal peripheral blood a variable percentage of cells express the CD69 antigen, and it is involved in lymphocyte signal transduction. Expression CD69 is induced upon activation of T lymphocytes, and may play a role in proliferation. Furthermore, the protein may act to transmit signals in natural killer cells and platelets. Alternative splicing results in multiple transcript variants of CD69. Diseases associated with CD69 dysfunction include coccidiodomycosis and asthma.仅用于科研。不用于诊断过程。未经明确授权不得转售。

数据

CD69 Antibody (17-0691-82) in FlowStaining of 1-day ConA-stimulated BALB/c splenocytes with 0.125 µg of Armenian Hamster IgG Isotype Control APC(Product # 17-4888-82) (blue histogram) or 0.125 µg of Anti-Mouse CD69 APC (purple histogram). Total viable cells were used for analysis.

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参考文献：

1. Acta pharmacologica SinicaBetulin from Hedyotis hedyotidea ameliorates concanavalin A-induced and T cell-mediated autoimmune hepatitis in mice.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer2. JCI insightSerum Gp96 is a chaperone of complement-C3 during graft-versus-host disease."17-0691 was used in Flow cytometry/Cell sorting to identify glycoprotein 96 and complement C3 as partner graft-versus-host disease-induced tissue damage-associated molecular pattern molecules."AuthorsSeignez A,Joly AL,Chaumonnot K,Hazoumé A,Sanka M,Marcion G,Boudesco C,Hammann A,Seigneuric R,Jégo G,Ducoroy P,Delarue P,Senet P,Castilla-Llorente C,Solary E,Durey MA,Rubio MT,Hermine O,Kohli E,Garrido C3. Frontiers in immunologyMicroRNA-7 Deficiency Ameliorates the Pathologies of Acute Lung Injury through Elevating KLF4."17-0691 was used in Flow cytometry/Cell sorting to study the role of microRNA-7 in acute lung injury, allowing the development of new therapeutic strategies against inflammatory lung disease."AuthorsZhao J,Chen C,Guo M,Tao Y,Cui P,Zhou Y,Qin N,Zheng J,Zhang J,Xu L4. eLifeAffinity and dose of TCR engagement yield proportional enhancer and gene activity in CD4+ T cells.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer5. Nature communicationsAlternative splicing of MALT1 controls signalling and activation of CD4(+) T cells."17-0691 was used in Flow cytometry/Cell sorting to study how TCR-induced alternative splicing augments MALT1 scaffolding to enhance downstream signalling and to promote optimal T-cell activation."AuthorsMeininger I,Griesbach RA,Hu D,Gehring T,Seeholzer T,Bertossi A,Kranich J,Oeckinghaus A,Eitelhuber AC,Greczmiel U,Gewies A,Schmidt-Supprian M,Ruland J,Brocker T,Heissmeyer V,Heyd F,Krappmann D

技术参数

产品应用 Flow

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