CD45.1 Monoclonal Antibody (A20), APC

货号：17-0453-81,17-0453-82

规格：50μg,100μg

价格：1620,2934

产品类型：流式抗体

品牌：Thermo Fisher

物种：小鼠

宿主：小鼠

抗体亚型：IgG

克隆号：A20

荧光染料：APC

抗体类型:	单抗	同型对照：	Mouse IgG2a kappa Isotype Control, APC, eBioscience™
免疫原性：		用法：	0.5 µg/test(Flow）

Description: The A20 monoclonal antibody reacts with the mouse CD45 molecule, the leukocyte common antigen (LCA) in CD45.1-expressing mouse strains. The strains that express CD45.1 include SJL/J, DA, STS/A and RIII. CD45.1 is expressed by all leukocytes in these strains.Applications Reported: The A20 antibody has been reported for use in flow cytometric analysis.Applications Tested: The A20 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationCD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization

CD45.1 Antibody (17-0453-82) in FlowStaining of BALB/c (left) or SJL (right) splenocytes with staining buffer (autofluorescence) (open histogram) or 0.25 µg of Anti-Mouse CD45.1 APC (filled histogram). Total viable cells were used for analysis.

数据

CD45.1 Antibody (17-0453-82) in Flow Staining of BALB/c (left) or SJL (right) splenocytes with staining buffer (autofluorescence) (open histogram) or 0.25 µg of Anti-Mouse CD45.1 APC (filled histogram). Total viable cells were used for analysis.

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参考文献：

1. ImmunityEpidermal Growth Factor Receptor Expression Licenses Type-2 Helper T Cells to Function in a T Cell Receptor-Independent Fashion.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer2. The Journal of experimental medicineEgr2 and 3 control adaptive immune responses by temporally uncoupling expansion from T cell differentiation.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer3. Cell stem cellAdipocyte Accumulation in the Bone Marrow during Obesity and Aging Impairs Stem Cell-Based Hematopoietic and Bone Regeneration."17-0453 was used in Flow cytometry/Cell sorting to specify the cellular identities of the adipogenic and osteogenic lineages of the bone."AuthorsAmbrosi TH,Scialdone A,Graja A,Gohlke S,Jank AM,Bocian C,Woelk L,Fan H,Logan DW,Schürmann A,Saraiva LR,Schulz TJ4. Stem cell reportsMurine iPSC-Derived Macrophages as a Tool for Disease Modeling of Hereditary Pulmonary Alveolar Proteinosis due to Csf2rb Deficiency.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer5. OncoimmunologyC-C chemokine receptor type-4 transduction of T cells enhances interaction with dendritic cells, tumor infiltration and therapeutic efficacy of adoptive T cell transfer."17-0453 was used in Flow cytometry/Cell sorting to study how CCR4 overexpression in CTL is a promising therapeutic strategy to enhance the efficacy of adoptive T cell transfer (ACT)."AuthorsRapp M,Grassmann S,Chaloupka M,Layritz P,Kruger S,Ormanns S,Rataj F,Janssen KP,Endres S,Anz D,Kobold S

技术参数

产品应用 Flow

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