Goat anti-Mouse IgG (H+L) Secondary Antibody, AP/山羊抗小鼠IgG（H + L）二抗，AP

货号：31321

规格：0.2 mL

价格：1383

产品类型：荧光二抗

品牌：Thermo Fisher

抗原：Purified Mouse IgG, whole molecule

物种：小鼠

宿主：山羊

抗体亚型：IgG

荧光染料：AP(碱性磷酸酶)

类型:	二抗	同型对照：
浓度：	0.6 mg/mL	用法：	1:5,000-1:50,000(IHC)；1:500-1:5,000(IP)；1:5,000-1:50,000(WB)

产品详细信息Product # 31321 has been successfully used in Western blot, IHC and IP applications.Product # 31321 reacts with the heavy chains of mouse IgG and with the light chains common to most mouse immunoglobulins, but does not react against non-immunoglobulin serum proteins. However, this antibody may cross-react with immunoglobulins from other species.Store product at 4°C until opened. To extend the shelf-life of this product, add an equal volume of glycerol to make a final concentration of approximately 50% glycerol and store at -20°C.Reconstitute with 0.2 mL of distilled water (1 mg/mL after restoration).靶标信息Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondaryantibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

数据

Mouse IgG (H+L) Secondary Antibody (31321) in WBWestern blot analysis was performed on whole cell extracts (30 µg lysate) of Jurkat (Lane 1) and K-562 (Lane 2). The blots were probed with Anti-SOD2 Mouse Monoclonal Antibody (Product # MA1-106, 2 µg/mL) and detected by chemiluminescence of alkaline phosphatase (AP) using Goat anti-Mouse IgG (H+L) Secondary Antibody, AP conjugate (Product # 31321) at dilutions 1:5,000 (Fig. 1), 1:20,000 (Fig. 2), and 1:50,000 (Fig. 3). A 22 kDa band corresponding to SOD2 was observed. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary antibody after blocking with 5 % skimmed milk. Chemiluminescent detection of alkaline phosphatase (AP) was performed using Novex® AP Chemiluminescent Substrate (CDP-Star®) (Product # WP20002) with Novex® AP Chemiluminescent Substrate Enhancer (Nitro Block II™) (Product # WP20003).

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