Rat IgG2a kappa Isotype Control, Functional Grade
货号：16-4321-81,16-4321-82,16-4321-85
规格：50μg,100μg,500μg
价格：693,1156,2103
产品类型：大鼠同型对照
品牌：Thermo Fisher
物种：其它
宿主：大鼠
抗体亚型：IgG2a, kappa
克隆号：eBR2a
荧光染料：其它
抗体类型: | 大鼠同型对照 | 同型对照： | IgG2a, kappa |
浓度： | 1 mg/mL | 用法： | Assay-Dependent (Ctrl)；Assay-Dependent (Flow)；Assay-Dependent (FN) |
Description: The monoclonal rat IgG2a, kappa is useful as an isotype control immunoglobulin.Applications Reported: This rat IgG2a isotype control has been reported for use in flow cytometric analysis and ELISA.Applications Tested: This rat IgG2a isotype control has been used by flow cytometric analysis of mouse splenocyte suspension and can be used at the same concentration as the experimental antibody.Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.Background/Target InformationThe isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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参考文献： |
| 1. Nature communicationsTreg engage lymphotoxin beta receptor for afferent lymphatic transendothelial migration."16-4321 was used in Blocking experiments to demonstrate a form of T-cell migration used only by Treg in tissues that serves an important role in their suppressive function."AuthorsBrinkman CC,Iwami D,Hritzo MK,Xiong Y,Ahmad S,Simon T,Hippen KL,Blazar BR,Bromberg JS2. Nature communicationsThe HIF-1/glial TIM-3 axis controls inflammation-associated brain damage under hypoxia."16-4321 was used in Blocking experiments to investigate the role of TIM-3 in inflammation and damage following ischaemia."AuthorsKoh HS,Chang CY,Jeon SB,Yoon HJ,Ahn YH,Kim HS,Kim IH,Jeon SH,Johnson RS,Park EJ3. PloS oneDifferential effects of peptidoglycan recognition proteins on experimental atopic and contact dermatitis mediated by Treg and Th17 cells."16-4321 was used in a Neutralization assay to demonstrate that peptidoglycan recognition proteins (PGRP) modulate sensitivity to chemically-induced atopic dermatitis and contact dermatitis using PGRP-knockout mice."AuthorsPark SY,Gupta D,Kim CH,Dziarski R4. Journal of immunology (Baltimore, Md. : 1950)Peptidoglycan recognition protein Pglyrp2 protects mice from psoriasis-like skin inflammation by promoting regulatory T cells and limiting Th17 responses."16-4321 was used in Neutralization experiments to indicate that Pglyrp2 limits overactivation of Th17 cells, by promoting accumulation of regulatory T-cells at inflammation sites, protecting skin from an inflammatory response."AuthorsPark SY,Gupta D,Hurwich R,Kim CH,Dziarski R |
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