Armenian Hamster IgG Isotype Control, PE-Cyanine5
货号：15-4888-82
规格：100μg
价格：2127
产品类型：同型对照
品牌：eBioscience
物种：其它
宿主：仓鼠
抗体亚型：IgG
克隆号：eBio299Arm
荧光染料：PE-Cy5
抗体类型: | 仓鼠同型对照 | 同型对照： | IgG |
浓度： | 0.2mg/mL | 用法： | Assay-Dependent (Ctrl)；Assay-Dependent (Flow) |
Description: The eBio299Arm monoclonal antibody is useful as an isotype control immunoglobulin.Applications Reported: This eBio299Arm antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBio299Arm antibody has been tested by flow cytometric analysis. Isotype control should be used at the same concentration as experimental antibody.Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationThe isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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参考文献： |
| 1. Journal of immunology (Baltimore, Md. : 1950)TLR9 and MyD88 are crucial for the development of protective immunity to malaria."15-4888 was used in an ELISA assay to investigate TLR9 and MyD88 regulation of pro/anti-inflammatory cytokines in mice infected with Plasmodium yoelii."AuthorsGowda NM,Wu X,Gowda DC2. PloS oneThe nucleosome (histone-DNA complex) is the TLR9-specific immunostimulatory component of Plasmodium falciparum that activates DCs."15-4888 was used in Flow cytometry/Cell sorting to provide a significant insight into the activation of DCs by malaria parasites."AuthorsGowda NM,Wu X,Gowda DC |
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