CD135 (Flt3) Monoclonal Antibody (A2F10), PE-Cyanine5, eBioscience™

货号：15-1351-81,15-1351-82

规格：50μg,100μg

价格：2000,3360

产品类型：流式抗体

品牌：Thermo Fisher

物种：小鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：A2F10

荧光染料：PE-Cy5

抗体类型:	单抗	同型对照：	Rat IgG2a kappa Isotype Control, PE-Cyanine5, eBioscience™
免疫原性：		用法：	5uL（1 µg）/test(Flow）

Description: The A2F10 monoclonal antibody reacts with mouse CD135, also known as Flk2/Flt3. A member of the tyrosine kinase receptor family, this 135-150 kDa molecule is expressed by primitive progenitor cells in fetal liver and adult bone marrow. Two-color staining of adult mouse bone marrow cells with A2F10 and RA3-6B2 (CD45R/B220) or M1/70 (CD11b) reveals Flk-2-positive subpopulations of B cells or myeloid cells, respectively.Applications Reported: This A2F10 antibody has been reported for use in flow cytometric analysis.Applications Tested: This A2F10 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationThis gene encodes a class III receptor tyrosine kinase that regulates hematopoiesis. The receptor consists of an extracellular domain composed of five immunoglobulin-like domains, one transmembrane region, and a cytoplasmic kinase domain split into two parts by a kinase-insert domain. The receptor is activated by binding of the fms-related tyrosine kinase 3 ligand to the extracellular domain, which induces homodimer formation in the plasma membrane leading to autophosphorylation of the receptor. The activated receptor kinase subsequently phosphorylates and activates multiple cytoplasmic effector molecules in pathways involved in apoptosis, proliferation, and differentiation of hematopoietic cells in bone marrow. Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia and acute lymphoblastic leukemia.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

CD135 (Flt3) Antibody (15-1351-82) in Flow Staining of BALB/c bone marrow cells with Anti-Mouse CD45-1 FITC (Product # 11-0453-82) and 0.5 µg of Anti-Mouse CD135 (Flt3) PE-Cyanine5.Total viable cells were used for analysis.

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参考文献：

1. Journal of cellular physiologyLCN2 overexpression in bone enhances the hematopoietic compartment via modulation of the bone marrow microenvironment."15135182 was used in flow cytometry to examine the function of lipocalin-2 in bone tissue"AuthorsCosta D,Principi E,Lazzarini E,Descalzi F,Cancedda R,Castagnola P,Tavella S2. eLifeClec11a/osteolectin is an osteogenic growth factor that promotes the maintenance of the adult skeleton."15-1351 was used in Flow cytometry/Cell sorting to study how Clec11a/Osteolectin maintains the adult skeleton by promoting the differentiation of mesenchymal progenitors into mature osteoblasts."AuthorsYue R,Shen B,Morrison SJ3. Experimental hematologyIndex sorting resolves heterogeneous murine hematopoietic stem cell populations."15-1351 was used in Flow cytometry/Cell sorting to indicate that when used in combination with single-cell functional assays, index sorting is a powerful tool for refining cell isolation strategies."AuthorsSchulte R,Wilson NK,Prick JC,Cossetti C,Maj MK,Gottgens B,Kent DG4. NatureOestrogen increases haematopoietic stem-cell self-renewal in females and during pregnancy."15-1351 was used in Flow cytometry/Cell sorting to assess the effect of oestradiol administration on division of haematopoietic stem cells in males and females."AuthorsNakada D,Oguro H,Levi BP,Ryan N,Kitano A,Saitoh Y,Takeichi M,Wendt GR,Morrison SJ5. BloodHIF prolyl hydroxylase 2 (PHD2) is a critical regulator of hematopoietic stem cell maintenance during steady-state and stress."15-1351 was used in Flow cytometry/Cell sorting to study the role of PHD2 in HSC compartment maintenance."AuthorsSingh RP,Franke K,Kalucka J,Mamlouk S,Muschter A,Gembarska A,Grinenko T,Willam C,Naumann R,Anastassiadis K,Stewart AF,Bornstein S,Chavakis T,Breier G,Waskow C,Wielockx

技术参数

产品应用 Flow

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