IL-6 Monoclonal Antibody (MP5-20F3), eBioscience™

货号：14-7061-81,14-7061-85

规格：50μg,500μg

价格：1264,3395

产品类型：ELISA检测

品牌：Thermo Fisher

物种：小鼠

宿主：大鼠

抗体亚型：IgG

克隆号：MP5-20F3

荧光染料：其它

抗体类型:	单抗	同型对照：	IgG1
免疫原性：		用法：	0.5-2 µg/mL（ELSA）

Description: The MP5-20F3 antibody reacts with mouse interleukin-6 (IL-6). The MP5-20F3 antibody is a neutralizing antibody. Mouse IL-6 (also known as B Cell Stimulating Factor, BCSF, and BSF-2) is a 21-28 kDa factor produced by a variety of cell types, including monocytes, fibroblasts and endothelial cells. Upon stimulation, macrophages, T, B, mast, and glial cells, eosinophils, keratinocytes and granulocytes also secrete IL-6. This pleiotrophic cytokine influences the antigen-specific immune response, inflammatory response, and the development of neurons, and is a major mediator of the acute phase reaction.Applications Reported: This MP5-20F3 antibody has been reported for use in ELISA capture antibody and ELISPOT capture antibody.Applications Tested: The MP5-20F3 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of mouse Interleukin-6 (IL-6) in combination with the biotin MP5-32C11 (cat. 13-7062) antibody for detection and recombinant mouse IL-6 (cat. 14-8061) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 0.5-2 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 8-1000 pg/mL should be included in each ELISA plate.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationInterleukin 6 (IL-6) is a multifunctional 26 kD protein originally discovered in the medium of RNA-stimulated fibroblastoid cells. IL-6 appears to be directly involved in the responses that occur after infection and cellular injury, and it may prove to be important as IL-1 and TNF-a in regulating the acute phase response. IL-6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages and endothelial cells. IL-6 acts upon a variety of cells including myeloid progenitor cells, T cells, B cells, and hepatocytes. In addition, IL-6 appears to interact with IL-2 in the proliferation of T lymphocytes. IL-6 potentiates the proliferative effect of IL-3 on multipotential hematopoietic progenitors. IL-6 plays a critical role in B-cell differentiation to plasma cells and is a potent growth factor for plasmacytoma and myeloma. IL-6 is a very useful culture supplement for the generation of a high number of antibody-producing hybridomas. Primarily produced at sites of acute and chronic inflammation, IL-6 is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of IL-6 is implicated in a wide variety of inflammation-associated disease states including diabetes mellitus and systemic juvenile rheumatoid arthritis.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

IL-6 Antibody (14-7061-85) in ELISA Mouse splenocytes (500,000 cells/ well) were activated with LPS (1 µg/mL) for 48 hours in Mouse IL-6 ELISPOT assay. Control well is medium alone.

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参考文献：

1. Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and MetabolismDepletion of microglia exacerbates postischemic inflammation and brain injury.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer2. Journal of leukocyte biologyγδ T cells promote inflammation and insulin resistance during high fat diet-induced obesity in mice."14-7061 was used in Flow cytometry/Cell sorting to reveal that isolated γδ T cells directly stimulated RAW264.7 macrophage TNF-α expression but did not stimulate inflammatory mediator expression in 3T3-L1 adipocytes."AuthorsMehta P,Nuotio-Antar AM,Smith CW3. Journal of immunology (Baltimore, Md. : 1950)Ly6C(high) monocytes control cerebral toxoplasmosis."14-7061 was used in Flow cytometry/Cell sorting to indicate the critical importance of recruited Ly6C monocytes upon cerebral toxoplasmosis."AuthorsBiswas A,Bruder D,Wolf SA,Jeron A,Mack M,Heimesaat MM,Dunay IR4. PloS oneNicotinic receptor alpha7 expression identifies a novel hematopoietic progenitor lineage."14-7061 was used in Flow cytometry/Cell sorting to investigate how inflammatory responses are mechanistically modulated by nicotinic acetylcholine receptors."AuthorsGahring LC,Enioutina EY,Myers EJ,Spangrude GJ,Efimova OV,Kelley TW,Tvrdik P,Capecchi MR,Rogers SW5. European journal of immunologyCytip regulates dendritic-cell function in contact hypersensitivity."14-7061 was used in Flow cytometry/Cell sorting to evaluate the functional consequences of cytohesin-interacting protein (Cytip) deficiency in dendritic cells using Cytip knockout mice."AuthorsHeib V,Sparber F,Tripp CH,Ortner D,Stoitzner P,Heufler C

技术参数

产品应用 ELSA

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