CD4 Monoclonal Antibody (GK1.5), PE-Cyanine5

货号：15-0041-81,15-0041-82,15-0041-83

规格：50μg,100μg,200μg

价格：1363,2059,4139

产品类型：流式抗体

品牌：Thermo Fisher

物种：小鼠

宿主：大鼠

抗体亚型：IgG2b, kappa

克隆号：GK1.5

荧光染料：PE-Cy5

抗体类型:	单抗	同型对照：	Rat IgG2b kappa Isotype Control, PE-Cyanine5, eBioscience™
免疫原性：		用法：	0.06 µg/test(Flow）

Description: The GK1.5 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by a majority of thymocytes, subpopulation of mature T cells and dendritic cells. CD4 binds to MHC class II on the surface of antigen presenting cells and plays an important role both in T cell development and in optimal functioning of mature T cells. In T cells, CD4 associates with protein tyrosine kinase p56lck through its cytoplasmic tail. Binding of GK1.5 is blocked by RM4-5.Applications Reported: The GK1.5 antibody has been reported for use in flow cytometric analysis.Applications Tested: The GK1.5 antibody has been tested by flow cytometric analysis of mouse thymocytes and splenocytes. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationThe CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017].For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

CD4 Antibody (15-0041-82) in Flow Staining of C57Bl/6 splenocytes with staining buffer (autofluorescence) (open histogram) or 0.03 µg of Anti-Mouse CD4 PE-Cyanine5 (filled histogram).Total cells were used for analysis

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参考文献：

1. mSphereMicrobiota Reconstitution Does Not Cause Bone Loss in Germ-Free Mice.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer2. Frontiers in pharmacologyTraditional Herbal Medicine-Derived Sulforaphene LFS-01 Reverses Colitis in Mice by Selectively Altering the Gut Microbiota and Promoting Intestinal Gamma-Delta T Cells....By clicking the link above, you will be redirected to the Benchsci website.Disclaimer3. Frontiers in physiologyMacrophage Depletion Protects against Cigarette Smoke-Induced Inflammatory Response in the Mouse Colon and Lung.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer4. PloS oneRottlerin as a therapeutic approach in psoriasis: Evidence from in vitro and in vivo studies.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer5. JCI insightIrgm1 coordinately regulates autoimmunity and host defense at select mucosal surfaces.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer

技术参数

产品应用 Flow

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