CD45R (B220) Monoclonal Antibody (RA3-6B2)

货号：14-0452-81,14-0452-82,14-0452-85,14-0452-86

规格：50μg,100μg,500μg,1mg

价格：582,914,2519,4013

产品类型：流式抗体

品牌：Thermo Fisher

物种：人/小鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：RA3-6B2

荧光染料：其它

抗体类型:	单抗	同型对照：	IgG2a, kappa
免疫原性：		用法：	1 µg/test(Flow)；5 µg/mL(IHC (F))；5 µg/mL(IHC (P))；Assay-Dependent(IP)

Description: The RA3-6B2 monoclonal antibody reacts with exon A-restricted isoform of mouse CD45, a 220 kDa surface molecule. CD45R/B220 epitope is mainly expressed by the B cell lineage from early Pro-B to mature B cells. However, some activated T cells, lymphokine activated killer cells (LAK), NK cell progenitors in the bone marrow, and T cells of the lpr/lpr mutant mouse also express this antigen.Applications Reported: The RA3-6B2 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining of frozen or formalin-fixed paraffin embedded tissue sections. RA3-6B2 has also been reported in functional studies of B cells. (Please use Functional Grade purified RA3-6B2, cat. 16-0452, in functional assays.).Applications Tested: The RA3-6B2 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. The RA3-6B2 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded mouse tissue using low and high pH antigen retrieval and can be used at less than or equal to 5 µg/mL. The RA3-6B2 antibody has been tested by immunohistochemistry of frozen mouse tissue and can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationCD45R is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. CD45R contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains. It is specifically expressed in hematopoietic cells and has been shown to be an essential regulator of T- and B-cell antigen receptor signaling.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

CD45R (B220) Antibody (14-0452-81) in IHC (P) Immunohistochemistry of formalin-fixed paraffin embedded mouse spleen using 5 µg/mL Anti-Human/Mouse CD45R (B220) Purified followed by Anti-Rat IgG Biotin, Avidin HRP, and DAB visualization. Nuclei are counterstained with hematoxylin.

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参考文献：

1. Frontiers in immunologyProapoptotic BIM Impacts B Lymphoid Homeostasis by Limiting the Survival of Mature B Cells in a Cell-Autonomous Manner.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer2. JCI insightIrgm1 coordinately regulates autoimmunity and host defense at select mucosal surfaces.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer3. PloS oneMultiple functional therapeutic effects of TnP: A small stable synthetic peptide derived from fish venom in a mouse model of multiple sclerosis....By clicking the link above, you will be redirected to the Benchsci website.Disclaimer4. Nature communicationsEZH2 enables germinal centre formation through epigenetic silencing of CDKN1A and an Rb-E2F1 feedback loop.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer5. Nature cell biologyDifferential cytokine contributions of perivascular haematopoietic stem cell niches.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer

技术参数

产品应用 Flow;IP

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